TY  - JOUR
A1  - Viñas, Jose Luis
A1  - Ventayol, Marina
A1  - Brüne, Bernhard
A1  - Jung, Michaela
A1  - Sola, Anna
A1  - Pi, Felip
A1  - Mastora, Chrysoula
A1  - Hotter, Georgina
T1  - miRNA let-7e modulates the Wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation
T2  - PLoS One
N2  - This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3βP) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3βP and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production.
Y1  - 2013
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/29747
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-297474
SN  - 1932-6203
N1  - Copyright: © 2013 Viñas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 8
IS  - (4): e60937
PB  - PLoS [u.a.]
CY  - Lawrence, Kan.
ER  -