TY  - INPR
A1  - Mishra, Bagdevi
A1  - Ulaszewski, Bartosz
A1  - Meger, Joanna
A1  - Aury, Jean-Marc
A1  - Bodénès, Catherine
A1  - Lesur-Kupin, Isabelle
A1  - Pfenninger, Markus
A1  - Da Silva, Corinne
A1  - Gupta, Deepak Kumar
A1  - Guichoux, Erwan
A1  - Heer, Katrin
A1  - Lalanne, Céline
A1  - Labadie, Karine
A1  - Opgenoorth, Lars
A1  - Ploch, Sebastian
A1  - Le Provost, Grégoire
A1  - Salse, Jérôme
A1  - Scotti, Ivan
A1  - Wötzel, Stefan
A1  - Plomion, Christophe
A1  - Burczyk, Jaroslaw
A1  - Thines, Marco
T1  - A chromosome-level genome assembly of the European Beech (Fagus sylvatica) reveals anomalies for organelle DNA integration, repeat content and distribution of SNPs
T2  - bioRxiv
N2  - The European Beech is the dominant climax tree in most regions of Central Europe and valued for its ecological versatility and hardwood timber. Even though a draft genome has been published recently, higher resolution is required for studying aspects of genome architecture and recombination. Here we present a chromosome-level assembly of the more than 300 year-old reference individual, Bhaga, from the Kellerwald-Edersee National Park (Germany). Its nuclear genome of 541 Mb was resolved into 12 chromosomes varying in length between 28 Mb and 73 Mb. Multiple nuclear insertions of parts of the chloroplast genome were observed, with one region on chromosome 11 spanning more than 2 Mb of the genome in which fragments up to 54,784 bp long and covering the whole chloroplast genome were inserted randomly. Unlike in Arabidopsis thaliana, ribosomal cistrons are present in Fagus sylvatica only in four major regions, in line with FISH studies. On most assembled chromosomes, telomeric repeats were found at both ends, while centromeric repeats were found to be scattered throughout the genome apart from their main occurrence per chromosome. The genome- wide distribution of SNPs was evaluated using a second individual from Jamy Nature Reserve (Poland). SNPs, repeat elements and duplicated genes were unevenly distributed in the genomes, with one major anomaly on chromosome 4. The genome presented here adds to the available highly resolved plant genomes and we hope it will serve as a valuable basis for future research on genome architecture and for understanding the past and future of European Beech populations in a changing climate.
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72979
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-729799
IS  - 2021.03.22.436437
ER  -