TY  - INPR
A1  - Oppold, Ann-Marie
A1  - Schmidt, Hanno
A1  - Rose, Marcel
A1  - Hellmann, Sören Lukas
A1  - Dolze, Florian
A1  - Ripp, Fabian
A1  - Weich, Bettina
A1  - Schmidt-Ott, Urs
A1  - Schmidt, Erwin
A1  - Kofler, Robert
A1  - Hankeln, Thomas
A1  - Pfenninger, Markus
T1  - Chironomus riparius (Diptera) genome sequencing reveals the impact of minisatellite transposable elements on population divergence
T2  - bioRxiv
N2  - Active transposable elements (TEs) may result in divergent genomic insertion and abundance patterns among conspecific populations. Upon secondary contact, such divergent genetic backgrounds can theoretically give rise to classical Dobzhansky-Muller incompatibilities (DMI), a way how TEs can contribute to the evolution of endogenous genetic barriers and eventually population divergence. We investigated whether differential TE activity created endogenous selection pressures among conspecific populations of the non-biting midge Chironomus riparius, focussing on a Chironomus-specific TE, the minisatellite-like Cla-element, whose activity is associated with speciation in the genus. Using an improved and annotated draft genome for a genomic study with five natural C. riparius populations, we found highly population-specific TE insertion patterns with many private insertions. A highly significant correlation of pairwise population FST from genome-wide SNPs with the FST estimated from TEs suggests drift as the major force driving TE population differentiation. However, the significantly higher Cla-element FST level due to a high proportion of differentially fixed Cla-element insertions indicates that segregating, i.e. heterozygous insertions are selected against. With reciprocal crossing experiments and fluorescent in-situ hybridisation of Cla-elements to polytene chromosomes, we documented phenotypic effects on female fertility and chromosomal mispairings that might be linked to DMI in hybrids. We propose that the inferred negative selection on heterozygous Cla-element insertions causes endogenous genetic barriers and therefore acts as DMI among C. riparius populations. The intrinsic genomic turnover exerted by TEs, thus, may have a direct impact on population divergence that is operationally different from drift and local adaptation.
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72417
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-724174
IS  - 080721
ER  -