TY  - JOUR
A1  - Buss, Stephan
A1  - Dobra, Jadranka
A1  - Goerg, Kerstin
A1  - Hoffmann, Stephanie
A1  - Kippenberger, Stefan
A1  - Kaufmann, Roland
A1  - Hofmann, Matthias
A1  - Bernd, August
T1  - Visible light is a better co-inducer of apoptosis for curcumin-treated human melanoma cells than UVA
T2  - PLoS One
N2  - Curcumin attracts worldwide scientific interest due to its anti-proliferative and apoptosis inducing effects on different tumor cells at concentrations ranging from 10 to 150 µM (3.7–55 µg/ml). Unfortunately, because of a low oral bioavailability, only low and pharmacologically ineffective serum levels are achievable. In this study, an alternative treatment concept consisting of low concentration curcumin (0.2–5 µg/ml) and irradiation with UVA or visible light (VL) has been tested. The experimental results show clearly that this treatment decreases the proliferation and the viability of human melanoma cells while the cell membrane integrity remains intact. We identified the onset of apoptosis characterized by typical markers such as active caspases 8, 9 and 3 as well as DNA fragmentation accompanied by the loss of cell adhesion. The mitochondrial apoptosis signaling pathway is predominant due to an early activation of caspase-9. The present data indicate a higher efficacy of a combination of curcumin and VL than curcumin and UVA. Reduced effects as a result of light absorption by heavily pigmented skin are unlikely if VL is used. These results indicate that a combination of curcumin and light irradiation may be a useful additional therapy in the treatment of malignant disease.
Y1  - 2013
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/32362
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-323628
SN  - 1932-6203
N1  - Copyright: © 2013 Buss et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 8
IS  - (11):e79748
PB  - PLoS
CY  - Lawrence, Kan.
ER  -