TY  - JOUR
A1  - Akbalik, Güney
A1  - Langebeck-Jensen, Kasper
A1  - Tushev, Georgi
A1  - Sambandan, Sivakumar
A1  - Rinne, Jennifer
A1  - Epstein, Irina
A1  - Cajigas, Ivan
A1  - Vlatkovic, Irena
A1  - Schuman, Erin
T1  - Visualization of newly synthesized neuronal RNA in vitro and in vivo using click-chemistry
T2  - RNA Biology
N2  - The neuronal transcriptome changes dynamically to adapt to stimuli from the extracellular and intracellular environment. In this study, we adapted for the first time a click chemistry technique to label the newly synthesized RNA in cultured hippocampal neurons and intact larval zebrafish brain. Ethynyl uridine (EU) was incorporated into neuronal RNA in a time- and concentration-dependent manner. Newly synthesized RNA granules observed throughout the dendrites were colocalized with mRNA and rRNA markers. In zebrafish larvae, the application of EU to the swim water resulted in uptake and labeling throughout the brain. Using a GABA receptor antagonist, PTZ (pentylenetetrazol), to elevate neuronal activity, we demonstrate that newly transcribed RNA signal increased in specific regions involved in neurogenesis.
KW  - Nascent RNA
KW  - neuronal RNA
KW  - newly synthesized RNA
KW  - PABP
KW  - RNA in zebrafish brain
KW  - visualization of RNA
KW  - Y10B
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/43924
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-439249
N1  - This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
VL  - 14
IS  - 1
SP  - 20
EP  - 28
PB  - Taylor and Francis
ER  -