Phage display selection of HIV specific conserved mimotopes with IgG from long-term non-progressors

  • Poster presentation Background The aim of this study is to identify conserved epitopes of HIV-1 neutralizing antibodies in polyclonal plasma from LTNP to finally derive vaccine candidates. Materials and methods The presence of neutralizing antibodies in 9 LTNP sera was proved by in vitro neutralization assays. Phage displayed peptide libraries were screened with LTNP IgG. HIV-specific mimotopes were analyzed for homology to the gp120 structure by a software (3DEX) especially developed for this purpose. Mice were immunized with interesting phages and their sera were analyzed for neutralizing activities against HIV-1. Results After biopannings, between 19% and 75% HIV-specific phage clones were identified by ELISA. Mimotope sequences were identified and could be aligned by 3DEX to linear or conformational epitopes on gp120. A peptide specific immune response was detected in sera of immunized mice. The first mice sera analyzed showed neutralizing activities against HIV-1. Conclusion Mimotopes could be selected from LTNP sera that represent conformational epitopes on gp120. Those ones inducing neutralizing antibodies upon immunization potentially are suited to derive vaccine candidates.

Download full text files

Export metadata

Metadaten
Author:Michael Humber, Sascha Antoni, Andreas Schreiber, Berta Rodes, Vicente Soriano, Matthias Dittmar, Ursula Dietrich
URN:urn:nbn:de:hebis:30-44519
DOI:https://doi.org/10.1186/1742-4690-2-S1-P29
ISSN:1742-4690
Parent Title (English):Retrovirology
Publisher:BioMed Central
Place of publication:London
Document Type:Article
Language:English
Year of Completion:2005
Date of first Publication:2005/12/08
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2007/05/22
Volume:2
Issue:(Suppl 1):P29
Page Number:1
First Page:1
Last Page:1
Note:
licensee BioMed Central Ltd.
Source:Retrovirology 2005, 2(Suppl 1):P29. - 2005 International Meeting of The Institute of Human Virology, Baltimore, USA. 29 August – 2 September 2005. - doi:10.1186/1742-4690-2-S1-P29
HeBIS-PPN:188414290
Institutes:Angeschlossene und kooperierende Institutionen / Georg-Speyer-Haus
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoDeutsches Urheberrecht