TIM23-mediated insertion of transmembrane alpha-helices into the mitochondrial inner membrane

While overall hydrophobicity is generally recognized as the main characteristic of transmembrane (TM) alpha-helices, the only membrane system for which there are detailed quantitative data on how different amino acids co
While overall hydrophobicity is generally recognized as the main characteristic of transmembrane (TM) alpha-helices, the only membrane system for which there are detailed quantitative data on how different amino acids contribute to the overall efficiency of membrane insertion is the endoplasmic reticulum (ER) of eukaryotic cells. Here, we provide comparable data for TIM23-mediated membrane protein insertion into the inner mitochondrial membrane of yeast cells. We find that hydrophobicity and the location of polar and aromatic residues are strong determinants of membrane insertion. These results parallel what has been found previously for the ER. However, we see striking differences between the effects elicited by charged residues flanking the TM segments when comparing the mitochondrial inner membrane and the ER, pointing to an unanticipated difference between the two insertion systems. Keywords: CoxVa , membrane protein , Mgm1p , mitochondria , TIM23
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Metadaten
Author:Salomé Calado Botelho, Marie Österberg, Andreas S. Reichert, Koji Yamano, Patrik Björkholm, Toshiya Endo, Gunnar von Heijne, Hyun Kim
URN:urn:nbn:de:hebis:30-97828
Document Type:Article
Language:English
Date of Publication (online):2011/04/07
Year of first Publication:2011
Publishing Institution:Univ.-Bibliothek Frankfurt am Main
Release Date:2011/04/07
Tag:CoxVa ; Mgm1p ; TIM23; membrane protein ; mitochondria
Note:
This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial No Derivative Works 3.0 Unported License, which permits distribution and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.
Source:The EMBO Journal (2011) 30, 1003 - 1011 ; doi:10.1038/emboj.2011.29 ; http://www.nature.com/emboj/journal/v30/n6/abs/emboj201129a.html
HeBIS PPN:235707570
Institutes:Medizin
Dewey Decimal Classification:570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
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Licence (German):License Logo Veröffentlichungsvertrag für Publikationen

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