Kengo Tsuda, Tatsuhiko Someya, Kanako Kuwasako, Mari Takahashi, Fahu He, Satoru Unzai, Makoto Inoue, Takushi Harada, Satoru Watanabe, Takaho Terada, Naohiro Kobayashi, Mikako Shirouzu, Takanori Kigawa, Akiko Tanaka, Sumio Sugano, Peter Güntert, Shigeyuki Yokoyama, Yutaka Muto
- Human Transformer2-beta (hTra2-beta) is an important member of the serine/arginine-rich protein family, and contains one RNA recognition motif (RRM). It controls the alternative splicing of several pre-mRNAs, including those of the calcitonin/calcitonin gene-related peptide (CGRP), the survival motor neuron 1 (SMN1) protein and the tau protein. Accordingly, the RRM of hTra2-beta specifically binds to two types of RNA sequences [the CAA and (GAA)2 sequences]. We determined the solution structure of the hTra2-beta RRM (spanning residues Asn110–Thr201), which not only has a canonical RRM fold, but also an unusual alignment of the aromatic amino acids on the beta-sheet surface. We then solved the complex structure of the hTra2-beta RRM with the (GAA)2 sequence, and found that the AGAA tetra-nucleotide was specifically recognized through hydrogen-bond formation with several amino acids on the N- and C-terminal extensions, as well as stacking interactions mediated by the unusually aligned aromatic rings on the beta-sheet surface. Further NMR experiments revealed that the hTra2-beta RRM recognizes the CAA sequence when it is integrated in the stem-loop structure. This study indicates that the hTra2-beta RRM recognizes two types of RNA sequences in different RNA binding modes.
MetadatenAuthor: | Kengo Tsuda, Tatsuhiko Someya, Kanako KuwasakoORCiD, Mari Takahashi, Fahu He, Satoru Unzai, Makoto Inoue, Takushi Harada, Satoru Watanabe, Takaho Terada, Naohiro Kobayashi, Mikako Shirouzu, Takanori Kigawa, Akiko Tanaka, Sumio Sugano, Peter GüntertORCiDGND, Shigeyuki Yokoyama, Yutaka Muto |
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URN: | urn:nbn:de:hebis:30-85027 |
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DOI: | https://doi.org/10.1093/nar/gkq854 |
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ISSN: | 1362-4962 |
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ISSN: | 0305-1048 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/20926394 |
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Parent Title (English): | Nucleic acids research |
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Publisher: | Oxford Univ. Press |
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Place of publication: | Oxford |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2010/11/12 |
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Date of first Publication: | 2010/10/05 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2010/11/12 |
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Volume: | 39 |
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Issue: | 4 |
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Page Number: | 16 |
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First Page: | 1538 |
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Last Page: | 1553 |
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Note: | © The Author(s) 2010. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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Source: | Nucleic Acids Research (2010) ; doi: 10.1093/nar/gkq854 |
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HeBIS-PPN: | 230016847 |
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Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
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| Wissenschaftliche Zentren und koordinierte Programme / Frankfurt Institute for Advanced Studies (FIAS) |
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| Wissenschaftliche Zentren und koordinierte Programme / Zentrum für Biomolekulare Magnetische Resonanz (BMRZ) |
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Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Sammlungen: | Universitätspublikationen |
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| Sammlung Biologie / Sondersammelgebiets-Volltexte |
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Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell 3.0 |
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