Contribution of the infection-associated complement regulator-acquiring surface protein 4 (ErpC) to complement resistance of Borrelia burgdorferi

Borrelia burgdorferi evades complement-mediated killing by interacting with complement regulators through distinct complement regulator-acquiring surface proteins (CRASPs). Here, we extend our analyses to the contributio
Borrelia burgdorferi evades complement-mediated killing by interacting with complement regulators through distinct complement regulator-acquiring surface proteins (CRASPs). Here, we extend our analyses to the contribution of CRASP-4 in mediating complement resistance of B. burgdorferi and its interaction with human complement regulators. CRASP-4 (also known as ErpC) was immobilized onto magnetic beads and used to capture proteins from human serum. Following Western blotting, factor H (CFH), CFH-related protein 1 (CFHR1), CFHR2, and CFHR5 were identified as ligands of CRASP-4. To analyze the impact of native CRASP-4 on mediating survival of serum-sensitive cells in human serum, a B. garinii strain was generated that ectopically expresses CRASP-4. CRASP-4-producing bacteria bound CFHR1, CFHR2, and CFHR5 but not CFH. In addition, transformed spirochetes deposited significant amounts of lethal complement components on their surface and were susceptible to human serum, thus indicating that CRASP-4 plays a subordinate role in complement resistance of B. burgdorferi.
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Metadaten
Author:Claudia Hammerschmidt, Teresia Hallström, Christine Skerka, Reinhard Wallich, Brian Stevenson, Peter F. Zipfel, Peter Kraiczy
URN:urn:nbn:de:hebis:30:3-244645
DOI:http://dx.doi.org/10.1155/2012/349657
ISSN:1740-2530
ISSN:1740-2522
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=22400034
Parent Title (English):Clinical and developmental immunology
Publisher:Hindawi
Place of publication:New York, NY
Document Type:Article
Language:English
Year of Completion:2012
Date of first Publication:2012/01/30
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2012/05/31
Volume:2012
Issue:Article ID 349657
Pagenumber:12
HeBIS PPN:306526913
Institutes:Medizin
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 3.0

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