Protein C preserves microcirculation in a model of neonatal septic shock

Objectives: Sepsis remains a disease with a high mortality in neonates. Microcirculatory impairment plays a pivotal role in the development of multiorgan failure in septic newborns. The hemodynamic effects of recombinant
Objectives: Sepsis remains a disease with a high mortality in neonates. Microcirculatory impairment plays a pivotal role in the development of multiorgan failure in septic newborns. The hemodynamic effects of recombinant activated protein C (rhAPC) were tested in an animal model of neonatal septic shock focusing on intestinal microcirculation.

Materials and methods: Endotoxic shock was triggered by intravenous application of Escherichia coli lipopolysaccarides in newborn piglets. Thereafter, five animals received a continuous infusion of 24 µg/kg/h rhAPC, and five received vehicle for control. Over the course of three hours, intestinal microcirculation was assessed by intravital microscopy every 30 min. Macrocirculation and blood counts were monitored simultaneously.

Results: After a short hypotensive period in all animals, the arterial blood pressure returned to baseline in the rhAPC-treated piglets, whereas the hypotension became increasingly severe in the controls. By 90 min, mean blood pressure in the controls was significantly lower than in the treatment group. Similar observations were made regaring microcirculation. After an early impairment in all study animals, functional capillary density and intestinal microcirculatory red blood cell velocity and red blood cell flow recovered in the rhAPC group, but deteriorated further in the control piglets.

Conclusion: Recombinant activated protein C protects macro- and microcirculation from endotoxic shock.
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Metadaten
Author:Doris Fischer, Marcel Friedrich Nold, Claudia A. Nold-Petry, Antonio Furlan, Alex Veldman
URN:urn:nbn:de:hebis:30:3-255408
DOI:http://dx.doi.org/10.2147/VHRM.S5863
ISSN:1176-6344
ISSN:1178-2048
Parent Title (English):Vascular health and risk management
Publisher:Dove Medical Press
Place of publication:Albany, Auckland
Document Type:Article
Language:English
Year of first Publication:2005
Publishing Institution:Univ.-Bibliothek Frankfurt am Main
Release Date:2012/07/16
Tag:intravital microscopy; microcirculation; neonatal septic shock; protein C
Volume:5.2005
Pagenumber:7
First Page:775
Last Page:781
Institutes:Medizin
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell 3.0

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