Biosynthesis of fucoxanthin and diadinoxanthin and function of initial pathway genes in Phaeodactylum tricornutum

The biosynthesis pathway to diadinoxanthin and fucoxanthin was elucidated in Phaeodactylum tricornutum by a combined approach involving metabolite analysis identification of gene function. For the initial steps leading t
The biosynthesis pathway to diadinoxanthin and fucoxanthin was elucidated in Phaeodactylum tricornutum by a combined approach involving metabolite analysis identification of gene function. For the initial steps leading to β-carotene, putative genes were selected from the genomic database and the function of several of them identified by genetic pathway complementation in Escherichia coli. They included genes encoding a phytoene synthase, a phytoene desaturase, a ζ-carotene desaturase, and a lycopene β-cyclase. Intermediates of the pathway beyond β-carotene, present in trace amounts, were separated by TLC and identified as violaxanthin and neoxanthin in the enriched fraction. Neoxanthin is a branching point for the synthesis of both diadinoxanthin and fucoxanthin and the mechanisms for their formation were proposed. A single isomerization of one of the allenic double bounds in neoxanthin yields diadinoxanhin. Two reactions, hydroxylation at C8 in combination with a keto-enol tautomerization and acetylation of the 3′-HO group results in the formation of fucoxanthin.
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Metadaten
Author:Michael Dambek, Ulrike Eilers, Jürgen Breitenbach, Sabine Steiger, Claudia Büchel, Gerhard Sandmann
URN:urn:nbn:de:hebis:30:3-255945
ISSN:1460-2431
ISSN:0022-0957
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=22888128
Parent Title (English):The journal of experimental botany
Publisher:Univ. Press
Place of publication:Oxford
Document Type:Article
Language:English
Date of Publication (online):2012/08/09
Date of first Publication:2012/08/09
Publishing Institution:Univ.-Bibliothek Frankfurt am Main
Release Date:2012/08/15
Tag:Phaeodactylum tricornutum; carotenogenic genes; complementation; diadinoxanthin; fucoxanthin; genetic pathway; neoxanthin
Volume:63
Issue:ers211
Pagenumber:6
First Page:1
Last Page:6
Institutes:Biowissenschaften
Dewey Decimal Classification:580 Pflanzen (Botanik)
Sammlungen:Universitätspublikationen
Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell 3.0

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