Arrangement of electron transport chain components in bovine mitochondrial supercomplex I1III2IV1

The respiratory chain in the inner mitochondrial membrane contains three large multi-enzyme complexes that together establish the proton gradient for ATP synthesis, and assemble into a supercomplex. A 19-Å 3D map of the 
The respiratory chain in the inner mitochondrial membrane contains three large multi-enzyme complexes that together establish the proton gradient for ATP synthesis, and assemble into a supercomplex. A 19-Å 3D map of the 1.7-MDa amphipol-solubilized supercomplex I1III2IV1 from bovine heart obtained by single-particle electron cryo-microscopy reveals an amphipol belt replacing the membrane lipid bilayer. A precise fit of the X-ray structures of complex I, the complex III dimer, and monomeric complex IV indicates distances of 13 nm between the ubiquinol-binding sites of complexes I and III, and of 10–11 nm between the cytochrome c binding sites of complexes III and IV. The arrangement of respiratory chain complexes suggests two possible pathways for efficient electron transfer through the supercomplex, of which the shorter branch through the complex III monomer proximal to complex I may be preferred.
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Metadaten
Author:Thorsten Althoff, Deryck J Mills, Jean-Luc Popot, Werner Kühlbrandt
URN:urn:nbn:de:hebis:30:3-259298
DOI:http://dx.doi.org/10.1038/emboj.2011.324
ISSN:0261-4189
ISSN:1460-2075
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=21909073
Parent Title (English):The EMBO journal
Publisher:Nature Publ. Group
Place of publication:London
Document Type:Article
Language:English
Date of Publication (online):2011/09/09
Date of first Publication:2011/09/09
Publishing Institution:Univ.-Bibliothek Frankfurt am Main
Release Date:2012/08/30
Tag:amphipols; electron cryo-microscopy; membrane protein complex; respiratory chain; single-particle averaging
Volume:30
Pagenumber:13
First Page:4652
Last Page:4664
Institutes:Biochemie und Chemie
Dewey Decimal Classification:570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung-Keine kommerzielle Nutzung-Weitergabe unter gleichen Bedingungen

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