Analysis of a hybrid TATA box binding protein originating from mesophilic and thermophilic donor organisms

The TATA Box Binding Protein (TBP) is a 20 kD protein that is essential and universally conserved in eucarya and archaea. Especially among archaea, organisms can be found that live below 0°C as well as organisms that gro
The TATA Box Binding Protein (TBP) is a 20 kD protein that is essential and universally conserved in eucarya and archaea. Especially among archaea, organisms can be found that live below 0°C as well as organisms that grow above 100°C. The archaeal TBPs show a high sequence identity and a similar structure consisting of α-helices and β-sheets that are arranged in a saddle-shape 2-symmetric fold. In previous studies, we have characterized the thermal stability of thermophilic and mesophilic archaeal TBPs by infrared spectroscopy and showed the correlation between the transition temperature (Tm) and the optimal growth temperature (OGT) of the respective donor organism. In this study, a “new” mutant TBP has been constructed, produced, purified and analyzed for a deeper understanding of the molecular mechanisms of thermoadaptation. The β-sheet part of the mutant consists of the TBP from Methanothermobacter thermoautotrophicus (OGT 65°C, MtTBP65) whose α-helices have been exchanged by those of Methanosarcina mazei (OGT 37°C, MmTBP37). The Hybrid-TBP irreversibly aggregates after thermal unfolding just like MmTBP37 and MtTBP65, but the Tm lies between that of MmTBP37 and MtTBP65 indicating that the interaction between the α-helical and β-sheet part of the TBP is crucial for the thermal stability. The temperature stability is probably encoded in the variable α-helices that interact with the highly conserved and DNA binding β-sheets.
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Metadaten
Author:Annette Kopitz, Jörg Soppa, Karin Hauser
URN:urn:nbn:de:hebis:30:3-270127
DOI:http://dx.doi.org/10.3233/SPE-2010-0434
ISSN:2314-4920
ISSN:2314-4939
Parent Title (English):Journal of Spectroscopy
Publisher:Hindawi Publ. Corp.
Place of publication:New York, NY [u.a.]
Document Type:Article
Language:English
Year of first Publication:2010
Publishing Institution:Univ.-Bibliothek Frankfurt am Main
Release Date:2012/11/01
Tag:TATA box binding protein; archaea; hybrid; infrared spectroscopy; protein stability; thermoadaptation
Volume:24
Issue:3-4
Pagenumber:5
First Page:233
Last Page:237
Note:
Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the   Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Institutes:Physik
Biowissenschaften
Dewey Decimal Classification:570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung 3.0

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