Unmasking a temperature-dependent effect of the P. anserina i-AAA protease on aging and development

Different molecular pathways involved in maintaining mitochondrial function are of fundamental importance to control cellular homeostasis. Mitochondrial i-AAA protease is part of such a surveillance system, and PaIAP is 
Different molecular pathways involved in maintaining mitochondrial function are of fundamental importance to control cellular homeostasis. Mitochondrial i-AAA protease is part of such a surveillance system, and PaIAP is the putative ortholog in the fungal aging model Podospora anserina. Here, we investigate the role of PaIAP in aging and development. Deletion of the gene encoding PaIAP resulted in a specific phenotype. When incubated at 27°C, spore germination and fruiting body formation are not different from that of the corresponding wild-type strain. Unexpectedly, the lifespan of the deletion strain is strongly increased. In contrast, cultivation at an elevated temperature of 37°C leads to impairments in spore germination and fruiting body formation and to a reduced lifespan. The higher PaIAP abundance in wild-type strains of the fungus grown at elevated temperature and the phenotype of the deletion strain unmasks a temperature-related role of the protein. The protease appears to be part of a molecular system that has evolved to allow survival under changing temperatures, as they characteristically occur in nature.
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Metadaten
Author:Andrea Weil, Karin Luce, Stefan Dröse, Ilka Wittig, Ulrich Brandt, Heinz D. Osiewacz
URN:urn:nbn:de:hebis:30:3-314958
DOI:http://dx.doi.org/10.4161/cc.10.24.18560
ISSN:1551-4005
ISSN:1538-4101
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=22134244
Parent Title (English):Cell cycle
Publisher:Landes Bioscience
Place of publication:Georgetown, Tex.
Document Type:Article
Language:English
Date of Publication (online):2013/09/06
Year of first Publication:2011
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2013/09/06
Tag:Podospora anserina; aging; i-AAA protease; mitochondria; protein quality control; temperature
Volume:10
Issue:24
Pagenumber:11
First Page:4280
Last Page:4290
Note:
© 2011 Landes Bioscience
HeBIS PPN:353136352
Institutes:Biowissenschaften
Medizin
Dewey Decimal Classification:570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Sondersammelgebiets-Volltexte
Licence (German):License Logo Veröffentlichungsvertrag für Publikationen

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