Prospective validation of immunological infiltrate for prediction of response to neoadjuvant chemotherapy in HER2-negative breast cancer : a substudy of the neoadjuvant GeparQuinto trial

  • Introduction: We have recently described an increased lymphocytic infiltration rate in breast carcinoma tissue is a significant response predictor for anthracycline/taxane-based neoadjuvant chemotherapy (NACT). The aim of this study was to prospectively validate the tumor-associated lymphocyte infiltrate as predictive marker for response to anthracycline/taxane-based NACT. Patients and Methods: The immunological infiltrate was prospectively evaluated in a total of 313 core biopsies from HER2 negative patients of the multicenter PREDICT study, a substudy of the neoadjuvant GeparQuinto study. Intratumoral lymphocytes (iTuLy), stromal lymphocytes (strLy) as well as lymphocyte-predominant breast cancer (LPBC) were evaluated by histopathological assessment. Pathological complete response (pCR) rates were analyzed and compared between the defined subgroups using the exact test of Fisher. Results: Patients with lymphocyte-predominant breast cancer (LPBC) had a significantly increased pCR rate of 36.6%, compared to non-LPBC patients (14.3%, p<0.001). LPBC and stromal lymphocytes were significantly independent predictors for pCR in multivariate analysis (LPBC: OR 2.7, p = 0.003, strLy: OR 1.2, p = 0.01). The amount of intratumoral lymphocytes was significantly predictive for pCR in univariate (OR 1.2, p = 0.01) but not in multivariate logistic regression analysis (OR 1.2, p = 0.11). Conclusion: Confirming previous investigations of our group, we have prospectively validated in an independent cohort that an increased immunological infiltrate in breast tumor tissue is predictive for response to anthracycline/taxane-based NACT. Patients with LPBC and increased stromal lymphocyte infiltration have significantly increased pCR rates. The lymphocytic infiltrate is a promising additional parameter for histopathological evaluation of breast cancer core biopsies.

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Author:Yasmin Issa-Nummer, Silvia Darb-Esfahani, Sibylle LoiblORCiDGND, Georg Kunz, Valentina Nekljudova, Iris Schrader, Bruno Valentin Sinn, Hans-Ullrich Ulmer, Ralf Kronenwett, Marianne Just, Thorsten KühnORCiD, Kurt Diebold, Michael UntchORCiDGND, Frank Holms, Jens-Uwe BlohmerORCiDGND, Jörg-Olaf Habeck, Manfred Dietel, Friedrich Overkamp, Petra Krabisch, Gunter von MinckwitzORCiDGND, Carsten Michael Denkert
URN:urn:nbn:de:hebis:30:3-328874
DOI:https://doi.org/10.1371/journal.pone.0079775
ISSN:1932-6203
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/24312450
Parent Title (English):PLoS One
Publisher:PLoS
Place of publication:Lawrence, Kan.
Document Type:Article
Language:English
Date of Publication (online):2013/12/02
Date of first Publication:2013/12/02
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2014/01/28
Volume:8
Issue:(12):e79775
Page Number:7
Note:
Copyright: © 2013 Issa-Nummer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
HeBIS-PPN:363677453
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 3.0