Chemically induced photoswitching of fluorescent probes : a general concept for super-resolution microscopy

We review fluorescent probes that can be photoswitched or photoactivated and are suited for single-molecule localization based super-resolution microscopy. We exploit the underlying photochemical mechanisms that allow ph
We review fluorescent probes that can be photoswitched or photoactivated and are suited for single-molecule localization based super-resolution microscopy. We exploit the underlying photochemical mechanisms that allow photoswitching of many synthetic organic fluorophores in the presence of reducing agents, and study the impact of these on the photoswitching properties of various photoactivatable or photoconvertible fluorescent proteins. We have identified mEos2 as a fluorescent protein that exhibits reversible photoswitching under various imaging buffer conditions and present strategies to characterize reversible photoswitching. Finally, we discuss opportunities to combine fluorescent proteins with organic fluorophores for dual-color photoswitching microscopy. 
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Metadaten
Author:Ulrike Endesfelder, Sebastian Malkusch, Benjamin Flottmann, Justine Mondry, Piotr Liguzinski, Peter J. Verveer, Mike Heilemann
URN:urn:nbn:de:hebis:30:3-348104
DOI:http://dx.doi.org/10.3390/molecules16043106
ISSN:1420-3049
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=21490558
Parent Title (English):Molecules
Publisher:Molecular Diversity Preservation International
Place of publication:Basel
Document Type:Article
Language:English
Date of Publication (online):2011/04/13
Date of first Publication:2011/04/13
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2014/10/27
Tag:PALM; dSTORM; fluorescent proteins; photoswitchable organic fluorophores; super-resolution
Volume:16
Issue:4
Pagenumber:13
First Page:3106
Last Page:3118
Note:
© 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
HeBIS PPN:366619772
Institutes:Biochemie und Chemie
Dewey Decimal Classification:540 Chemie und zugeordnete Wissenschaften
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 3.0

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