Effects of flavonoid-induced oxidative stress on anti-H5N1 influenza A virus activity exerted by baicalein and biochanin A

Background: Different flavonoids are known to interfere with influenza A virus replication. Recently, we showed that the structurally similar flavonoids baicalein and biochanin A inhibit highly pathogenic avian H5N1 infl
Background: Different flavonoids are known to interfere with influenza A virus replication. Recently, we showed that the structurally similar flavonoids baicalein and biochanin A inhibit highly pathogenic avian H5N1 influenza A virus replication by different mechanisms in A549 lung cells. Here, we investigated the effects of both compounds on H5N1-induced reactive oxygen species (ROS) formation and the role of ROS formation during H5N1 replication.
Findings: Baicalein and biochanin A enhanced H5N1-induced ROS formation in A549 cells and primary human monocyte-derived macrophages. Suppression of ROS formation induced by baicalein and biochanin A using the antioxidant N-acetyl-L-cysteine strongly increased the anti-H5N1 activity of both compounds in A549 cells but not in macrophages.
Conclusions: These findings emphasise that flavonoids induce complex pharmacological actions some of which may interfere with H5N1 replication while others may support H5N1 replication. A more detailed understanding of these actions and the underlying structure-activity relationships is needed to design agents with optimised anti-H5N1 activity.
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Metadaten
Author:Martin Michaelis, Patchima Sithisarn, Jindrich Cinatl
URN:urn:nbn:de:hebis:30:3-352812
DOI:http://dx.doi.org/10.1186/1756-0500-7-384
ISSN:1756-0500
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=24958200
Parent Title (English):BMC Research Notes
Publisher:BioMed Central
Place of publication:London
Document Type:Article
Language:English
Date of Publication (online):2014/10/31
Date of first Publication:2014/06/23
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2014/10/31
Tag:H5N1; N-acetyl-L-cysteine; antiviral; baicalein; biochanin A; reactive oxygen species
Volume:7
Issue:Art. 384
Pagenumber:6
First Page:1
Last Page:6
Note:
© 2014 Michaelis et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
HeBIS PPN:366632051
Institutes:Medizin
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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