PADI4 acts as a coactivator of Tal1 by counteracting repressive histone arginine methylation

The transcription factor Tal1 is a critical activator or repressor of gene expression in hematopoiesis and leukaemia. The mechanism by which Tal1 differentially influences transcription of distinct genes is not fully und
The transcription factor Tal1 is a critical activator or repressor of gene expression in hematopoiesis and leukaemia. The mechanism by which Tal1 differentially influences transcription of distinct genes is not fully understood. Here we show that Tal1 interacts with the peptidylarginine deiminase IV (PADI4). We demonstrate that PADI4 can act as an epigenetic coactivator through influencing H3R2me2a. At the Tal1/PADI4 target gene IL6ST the repressive H3R2me2a mark triggered by PRMT6 is counteracted by PADI4, which augments the active H3K4me3 mark and thus increases IL6ST expression. In contrast, at the CTCF promoter PADI4 acts as a repressor. We propose that the influence of PADI4 on IL6ST transcription plays a role in the control of IL6ST expression during lineage differentiation of hematopoietic stem/progenitor cells. These results open the possibility to pharmacologically influence Tal1 in leukaemia.
show moreshow less

Download full text files

Export metadata

  • Export Bibtex
  • Export RIS

Additional Services

    Share in Twitter Search Google Scholar
Metadaten
Author:Stephan Kolodziej, Olga Nikolaevna Lausen, Thomas Oellerich, Julia Herglotz, Ingo Backert, Nicole Kohrs, Estella Buscató Arsequell, Sandra Kerstin Wittmann, Gabriela Salinas-Riester, Halvard-Björn Bönig, Michael Karas, Hubert Serve, Ewgenij Proschak, Jörn Lausen
URN:urn:nbn:de:hebis:30:3-373900
DOI:http://dx.doi.org/10.1038/ncomms4995
ISSN:2041-1723
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=24874575
Parent Title (English):Nature Communications
Publisher:Nature Publishing Group
Place of publication:London
Document Type:Article
Language:English
Date of Publication (online):2014/05/29
Date of first Publication:2014/05/29
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2015/10/08
Volume:5
Issue:3995
Pagenumber:12
First Page:1
Last Page:12
Note:
This work is licensed under a Creative Commons Attribution- NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons. org/licenses/by-nc-nd/3.0/
HeBIS PPN:37144845X
Institutes:Pharmazie
Medizin
Georg-Speyer-Haus
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0

$Rev: 11761 $