Cryo-EM structure of respiratory complex I reveals a link to mitochondrial sulfur metabolism

Mitochondrial complex I is a 1MDa membrane protein complex with a central role in aerobic energy metabolism. The bioenergetic core functions are executed by 14 central subunits that are conserved from bacteria to man. De
Mitochondrial complex I is a 1MDa membrane protein complex with a central role in aerobic energy metabolism. The bioenergetic core functions are executed by 14 central subunits that are conserved from bacteria to man. Despite recent progress in structure determination, our understanding of the function of the ~30 accessory subunits associated with the mitochondrial complex is still limited. We have investigated the structure of complex I from the aerobic yeast Yarrowia lipolytica by cryo-electron microscopy. Our density map at 7.9Å resolution closely matches the 3.6-3.9Å X-ray structure of the Yarrowia lipolytica complex. However, the cryo-EM map indicated an additional subunit on the side of the matrix arm above the membrane surface, pointing away from the membrane arm. The density, which is not present in any previously described complex I structure and occurs in about 20 % of the particles, was identified as the accessory sulfur transferase subunit ST1. The Yarrowia lipolytica complex I preparation is active in generating H2S from the cysteine derivative 3-mercaptopyruvate, catalyzed by ST1. We thus provide evidence for a link between respiratory complex I and mitochondrial sulfur metabolism.
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Metadaten
Author:Edoardo D'Imprima, Deryck J. Mills, Kristian Kurt Parey, Ulrich Brandt, Werner Kühlbrandt, Volker Zickermann, Janet Vonck
URN:urn:nbn:de:hebis:30:3-418133
DOI:http://dx.doi.org/10.1016/j.bbabio.2016.09.014
ISSN:0005-2728
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=27693469
Parent Title (English):Biochimica et Biophysica Acta
Document Type:Article
Language:English
Year of Completion:2016
Year of first Publication:2016
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2016/10/26
Tag:Complex I; Cryo-electron microscopy; Electron transport chain; Mitochondria; NADH:ubiquinone oxidoreductase; Sulfur metabolism
Volume:1857
Issue:12
Pagenumber:8
First Page:1935
Last Page:1942
Note:
© 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
HeBIS PPN:426711335
Institutes:Medizin
Dewey Decimal Classification:570 Biowissenschaften; Biologie
610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell - Keine Bearbeitung 4.0

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