Clinical application of liquid biopsy in targeted therapy of metastatic colorectal cancer

Background. Colorectal cancers (CRC) shed DNA into blood circulation. There is growing evidence that the analysis of circulating tumor DNA can be effectively used for monitoring of disease, to track tumor heterogeneity a
Background. Colorectal cancers (CRC) shed DNA into blood circulation. There is growing evidence that the analysis of circulating tumor DNA can be effectively used for monitoring of disease, to track tumor heterogeneity and to evaluate response to treatment. Case Presentation. Here, we describe two cases of patients with advanced CRC. The first case is about a patient with no available tissue for analysis of RAS mutation status. Liquid biopsy revealed RAS-wild-type and the therapy with anti-EGFR (epidermal growth factor receptor) monoclonal antibody cetuximab could be initiated. In the second case, the mutational profile of a patient with initial wild-type RAS-status was continually tracked during the course of treatment. An acquired KRAS exon 3 mutation was detected. The number of KRAS mutated fragments decreased continuously after the discontinuation of the therapy with EGFR-specific antibodies. Conclusion. Liquid biopsy provides a rapid genotype result, which accurately reproduces the current mutation status of tumor tissue. Furthermore, liquid biopsy enables close monitoring of the onset of secondary resistance to anti-EGFR therapy.
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Metadaten
Author:Jörg Trojan, Susanne Klein-Scory, Christine Koch, Wolff Schmiegel, Alexander Baraniskin
URN:urn:nbn:de:hebis:30:3-429204
DOI:http://dx.doi.org/10.1155/2017/6139634
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=28232873
Parent Title (English):Case Reports in Oncological Medicine
Document Type:Article
Language:English
Year of Completion:2017
Date of first Publication:2017/01/23
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2017/03/01
Volume:2017
Issue:Article ID 6139634
Pagenumber:4
Note:
Copyright © 2017 Jörg Trojan et al. This is an open access article distributed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
HeBIS PPN:452460689
Institutes:Medizin
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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