Structure of a type IV pilus machinery in the open and closed state

Proteins of the secretin family form large macromolecular complexes, which assemble in the outer membrane of Gram-negative bacteria. Secretins are major components of type II and III secretion systems and are linked to e
Proteins of the secretin family form large macromolecular complexes, which assemble in the outer membrane of Gram-negative bacteria. Secretins are major components of type II and III secretion systems and are linked to extrusion of type IV pili (T4P) and to DNA uptake. By electron cryo-tomography of whole Thermus thermophilus cells, we determined the in situ structure of a T4P molecular machine in the open and the closed state. Comparison reveals a major conformational change whereby the N-terminal domains of the central secretin PilQ shift by ∼30 Å, and two periplasmic gates open to make way for pilus extrusion. Furthermore, we determine the structure of the assembled pilus.
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Metadaten
Author:Vicki A. M. Gold, Ralf Salzer, Beate Averhoff, Werner Kühlbrandt
URN:urn:nbn:de:hebis:30:3-440599
DOI:http://dx.doi.org/10.7554/eLife.07380
ISSN:2050-084X
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=25997099
Parent Title (English):eLife
Publisher:eLife Sciences Publications
Place of publication:Cambridge
Contributor(s):Stephen C. Harrison
Document Type:Article
Language:English
Date of Publication (online):2017/05/22
Year of first Publication:2015
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2017/05/22
Tag:DNA transporter; Thermus thermophilus; bacterial secretion; biophysics; electron cryo-tomography; structural biology; subtomogram averaging; type IV pilus
Volume:4
Issue:e07380
Pagenumber:12
First Page:1
Last Page:12
Note:
Copyright Gold et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
HeBIS PPN:424950669
Institutes:Biowissenschaften
MPI für Biophysik
Dewey Decimal Classification:570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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