Combinatorial biosynthesis of novel multi-hydroxy carotenoids in the red yeast xanthophyllomyces dendrorhous

The red yeast Xanthophyllomyces dendrorhous is an established platform for the synthesis of carotenoids. It was used for the generation of novel multi oxygenated carotenoid structures. This was achieved by a combinatoria
The red yeast Xanthophyllomyces dendrorhous is an established platform for the synthesis of carotenoids. It was used for the generation of novel multi oxygenated carotenoid structures. This was achieved by a combinatorial approach starting with the selection of a β-carotene accumulating mutant, stepwise pathway engineering by integration of three microbial genes into the genome and finally the chemical reduction of the resulting 4,4’-diketo-nostoxanthin (2,3,2’,3’-tetrahydroxy-4,4’-diketo-β-carotene) and 4-keto-nostoxanthin (2,3,2’,3’-tetrahydroxy-4-monoketo-β-carotene). Both keto carotenoids and the resulting 4,4’-dihydroxy-nostoxanthin (2,3,4,2’,3’,4’-hexahydroxy-β-carotene) and 4-hydroxy-nostoxanthin (2,3,4,2’3’-pentahydroxy-β-carotene) were separated by high-performance liquid chromatography (HPLC) and analyzed by mass spectrometry. Their molecular masses and fragmentation patterns allowed the unequivocal identification of all four carotenoids.
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Metadaten
Author:Hendrik Pollmann, Jürgen Breitenbach, Hendrik Wolff, Helge Björn Bode, Gerhard Sandmann
URN:urn:nbn:de:hebis:30:3-442321
DOI:http://dx.doi.org/10.3390/jof3010009
ISSN:2309-608X
Parent Title (English):Journal of Fungi
Publisher:MDPI
Place of publication:Basel
Contributor(s):Laurent Dufossé, Yanis Caro, Mireille Fouillaud
Document Type:Article
Language:English
Date of Publication (online):2017/06/22
Date of first Publication:2017/02/22
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2017/06/22
Tag:4,4’-dihydroxy-nostoxanthin; 4,4’-diketo-nostoxanthin; HPLC separation; MS-MS spectra; Xanthophyllomyces dendrorhous; carotenoid biosynthesis; genetic engineering
Volume:3
Issue:1, Art. 9
Pagenumber:10
First Page:1
Last Page:10
Note:
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
HeBIS PPN:423606816
Institutes:Biowissenschaften
Dewey Decimal Classification:570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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