A novel mode of ubiquitin recognition by the ubiquitin-binding zinc finger domain of WRNIP1

The ubiquitin-binding zinc finger (UBZ) is a type of zinc-coordinating β-β-α fold domain found mainly in proteins involved in DNA repair and transcriptional regulation. Here, we report the crystal structure of the UBZ do
The ubiquitin-binding zinc finger (UBZ) is a type of zinc-coordinating β-β-α fold domain found mainly in proteins involved in DNA repair and transcriptional regulation. Here, we report the crystal structure of the UBZ domain of Y-family DNA polymerase (pol) η and the crystal structure of the complex between the UBZ domain of Werner helicase-interacting protein 1 (WRNIP1) and ubiquitin, crystallized using the GFP fusion technique. In contrast to the pol η UBZ, which has been proposed to bind ubiquitin via its C-terminal α-helix, ubiquitin binds to a novel surface of WRNIP1 UBZ composed of the first β-strand and the C-terminal α-helix. In addition, we report the structure of the tandem UBZ domains of Tax1-binding protein 1 (TAX1BP1) and show that the second UBZ of TAX1BP1 binds ubiquitin, presumably in a manner similar to that of WRNIP1 UBZ. We propose that UBZ domains can be divided into at least two different types in terms of the ubiquitin-binding surfaces: the pol η type and the WRNIP1 type.
show moreshow less

Download full text files

  • application/pdf Suzuki_et_al-2016-The_FEBS_Journal.pdf (2427 KB)

Export metadata

  • Export Bibtex
  • Export RIS
Metadaten
Author:Nobuhiro Suzuki, Ahmed Rohaim, Ryuichi Kato, Ivan Dikic, Soichi Wakatsuki, Masato Kawasaki
URN:urn:nbn:de:hebis:30:3-443039
DOI:http://dx.doi.org/10.1111/febs.13734
ISSN:1742-4658
ISSN:1742-464X
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=27062441
Parent Title (English):The FEBS journal
Publisher:Wiley-Blackwell
Place of publication:Oxford [u. a.]
Document Type:Article
Language:English
Date of Publication (online):2017/06/26
Year of first Publication:2016
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2017/06/26
Tag:TAX1BP1; WRNIP1; green fluorescent protein; pol g; ubiquitin-binding zinc finger
Volume:283
Issue:11
Pagenumber:14
First Page:2004
Last Page:2017
Note:
© 2016 Federation of European Biochemical Societies
HeBIS PPN:423894889
Institutes:Medizin
Exzellenzcluster Makromolekulare Komplexe
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoArchivex. zur Lesesaalplatznutzung § 52b UrhG

$Rev: 11761 $