Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy

The turnover of endoplasmic reticulum (ER) ensures the correct biological activity of its distinct domains. In mammalian cells, the ER is degraded via a selective autophagy pathway (ER-phagy), mediated by two specific re
The turnover of endoplasmic reticulum (ER) ensures the correct biological activity of its distinct domains. In mammalian cells, the ER is degraded via a selective autophagy pathway (ER-phagy), mediated by two specific receptors: FAM134B, responsible for the turnover of ER sheets and SEC62 that regulates ER recovery following stress. Here, we identified reticulon 3 (RTN3) as a specific receptor for the degradation of ER tubules. Oligomerization of the long isoform of RTN3 is sufficient to trigger fragmentation of ER tubules. The long N-terminal region of RTN3 contains several newly identified LC3-interacting regions (LIR). Binding to LC3s/GABARAPs is essential for the fragmentation of ER tubules and their delivery to lysosomes. RTN3-mediated ER-phagy requires conventional autophagy components, but is independent of FAM134B. None of the other reticulon family members have the ability to induce fragmentation of ER tubules during starvation. Therefore, we assign a unique function to RTN3 during autophagy.
show moreshow less

Metadaten
Author:Paolo Grumati, Giulio Morozzi, Soraya Hölper, Muriel Mari, Marie-Lena I. E. Harwardt, Riqiang Yan, Stefan Müller, Fulvio Reggiori, Mike Heilemann, Ivan Dikic
URN:urn:nbn:de:hebis:30:3-447092
DOI:http://dx.doi.org/10.7554/eLife.25555.001
ISSN:2050-084X
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=28617241
Parent Title (English):eLife
Publisher:eLife Sciences Publications
Place of publication:Cambridge
Document Type:Article
Language:English
Date of Publication (online):2017/11/16
Date of first Publication:2017/06/15
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2017/11/16
Tag:Autophagy; Biochemistry; Cell biology; Endoplasmic reticulum; Research article; Reticulon; Rtn3; Selective autophagy
Volume:6
Issue:e25555
Pagenumber:32
First Page:1
Last Page:32
Note:
Copyright Grumati et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. 
HeBIS PPN:424951398
Institutes:Biochemie und Chemie
Medizin
Exzellenzcluster Makromolekulare Komplexe
Dewey Decimal Classification:570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0

$Rev: 11761 $