Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo‐tomography

We employed electron cryo‐tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation‐arrested ribosomes reveal the clustered organization of the TOM complex, corroborating earlier reports of 
We employed electron cryo‐tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation‐arrested ribosomes reveal the clustered organization of the TOM complex, corroborating earlier reports of localized translation. Ribosomes are shown to interact specifically with the TOM complex, and nascent chain binding is crucial for ribosome recruitment and stabilization. Ribosomes are bound to the membrane in discrete clusters, often in the vicinity of the crista junctions. This interaction highlights how protein synthesis may be coupled with transport. Our work provides unique insights into the spatial organization of cytosolic ribosomes on mitochondria.
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Metadaten
Author:Vicki A. M. Gold, Piotr Chroscicki, Piotr Bragoszewski, Agnieszka Chacinska
URN:urn:nbn:de:hebis:30:3-465779
DOI:http://dx.doi.org/10.15252/embr.201744261
ISSN:1469-3178
ISSN:1469-221X
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=28827470
Parent Title (English):EMBO reports
Publisher:EMBO Press ; Wiley
Place of publication:Heidelberg ; Hoboken, NJ [u. a.]
Document Type:Article
Language:English
Year of Completion:2017
Date of first Publication:2017/08/21
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2018/06/07
Tag:TOM complex; electron cryo-tomography; mitochondrial protein import; ribosomes; translation
Volume:18
Issue:10
Pagenumber:15
First Page:1786
Last Page:1800
Note:
License: This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
HeBIS PPN:434433993
Institutes:Biowissenschaften
MPI für Biophysik
Dewey Decimal Classification:570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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