FUSE binding protein 1 (FUBP1) expression is upregulated by T-cell acute lymphocytic leukemia protein 1 (TAL1) and required for efficient erythroid differentiation

During erythropoiesis, haematopoietic stem cells (HSCs) differentiate in successive steps of commitment and specification to mature erythrocytes. This differentiation process is controlled by transcription factors that e
During erythropoiesis, haematopoietic stem cells (HSCs) differentiate in successive steps of commitment and specification to mature erythrocytes. This differentiation process is controlled by transcription factors that establish stage- and cell type-specific gene expression. In this study, we demonstrate that FUSE binding protein 1 (FUBP1), a transcriptional regulator important for HSC self-renewal and survival, is regulated by T-cell acute lymphocytic leukaemia 1 (TAL1) in erythroid progenitor cells. TAL1 directly activates the FUBP1 promoter, leading to increased FUBP1 expression during erythroid differentiation. The binding of TAL1 to the FUBP1 promoter is highly dependent on an intact GATA sequence in a combined E-box/GATA motif. We found that FUBP1 expression is required for efficient erythropoiesis, as FUBP1-deficient progenitor cells were limited in their potential of erythroid differentiation. Thus, the finding of an interconnection between GATA1/TAL1 and FUBP1 reveals a molecular mechanism that is part of the switch from progenitor- to erythrocyte-specific gene expression. In summary, we identified a TAL1/FUBP1 transcriptional relationship, whose physiological function in haematopoiesis is connected to proper erythropoiesis.
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Metadaten
Author:Marlene Steiner, Lucas Schneider, Jasmin Yillah, Katharina Gerlach, Olga Nikolaevna Lausen, Annekarin Meyer, Alisa Maring, Halvard-Björn Bönig, Erhard Seifried, Martin Zörnig, Jörn Lausen
URN:urn:nbn:de:hebis:30:3-486870
DOI:http://dx.doi.org/10.1371/journal.pone.0210515
ISSN:1932-6203
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=30653565
Parent Title (English):PLoS one
Publisher:PLoS
Place of publication:Lawrence, Kan.
Contributor(s):Arun Rishi
Document Type:Article
Language:English
Year of Completion:2019
Date of first Publication:2019/01/17
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/01/22
Tag:Cell binding; Cell differentiation; Erythropoiesis; Gene expression; Luciferase; Sequence motif analysis; Transcription factors; Transcriptional control
Volume:14
Issue:(1): e0210515
Pagenumber:22
First Page:1
Last Page:22
Note:
Copyright: © 2019 Steiner et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
HeBIS PPN:446225347
Institutes:Pharmazie
Biowissenschaften
Medizin
Georg-Speyer-Haus
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Open-Access-Publikationsfonds:Medizin
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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