Gold nanoparticle-mediated delivery of molecules into primary human gingival fibroblasts using ns-laser pulses : a pilot study

Interaction of gold nanoparticles (AuNPs) in the vicinity of cells’ membrane with a pulsed laser (λ = 532 nm, τ = 1 ns) leads to perforation of the cell membrane, thereby allowing extracellular molecules to diffuse into 
Interaction of gold nanoparticles (AuNPs) in the vicinity of cells’ membrane with a pulsed laser (λ = 532 nm, τ = 1 ns) leads to perforation of the cell membrane, thereby allowing extracellular molecules to diffuse into the cell. The objective of this study was to develop an experimental setting to deliver molecules into primary human gingival fibroblasts (pHFIB-G) by using ns-laser pulses interacting with AuNPs (study group). To compare the parameters required for manipulation of pHFIB-G with those needed for cell lines, a canine pleomorphic adenoma cell line (ZMTH3) was used (control group). Non-laser-treated cells incubated with AuNPs and the delivery molecules served as negative control. Laser irradiation (up to 35 mJ/cm2) resulted in a significant proportion of manipulated fibroblasts (up to 85%, compared to non-irradiated cells: p < 0.05), while cell viability (97%) was not reduced significantly. pHFIB-G were perforated as efficiently as ZMTH3. No significant decrease of metabolic cell activity was observed up to 72 h after laser treatment. The fibroblasts took up dextrans with molecular weights up to 500 kDa. Interaction of AuNPs and a pulsed laser beam yields a spatially selective technique for manipulation of even primary cells such as pHFIB-G in high throughput.
show moreshow less

Metadaten
Author:Judith Krawinkel, Maria Leilani Torres-Mapa, Kristian Werelius, Alexander Heisterkamp, Stefan Rüttermann, Georgios Romanos, Susanne Gerhardt-Szép
URN:urn:nbn:de:hebis:30:3-505704
DOI:http://dx.doi.org/10.3390/ma9050397
ISSN:1996-1944
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=28773519
Parent Title (English):Materials
Publisher:MDPI
Place of publication:Basel
Contributor(s):Nicola Pugno
Document Type:Article
Language:English
Year of Completion:2016
Date of first Publication:2016/05/20
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/07/25
Tag:gold nanoparticles; human gingival fibroblasts; laser; laser based cell manipulation
Volume:9
Issue:5, Art. 397
Pagenumber:12
First Page:1
Last Page:12
Note:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
HeBIS PPN:452223946
Institutes:Medizin
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0

$Rev: 11761 $