In vitro migration and proliferation ("wound healing") potential of mesenchymal stromal cells generated from human CD271+ bone marrow mononuclear cells

Background: Emerging evidence indicates that mesenchymal stromal cells (MSCs) isolated from different tissue sources may be used in vivo as tissue restorative agents. To date, there is no evidence, however, on migration 
Background: Emerging evidence indicates that mesenchymal stromal cells (MSCs) isolated from different tissue sources may be used in vivo as tissue restorative agents. To date, there is no evidence, however, on migration and proliferation ("wound healing") potential of different subsets of MSCs. The main goal of this study was therefore to compare the in vitro "wound healing" capacity of MSCs generated from positively selected CD271+ bone marrow mononuclear cells (CD271-MSCs) and MSCs generated by plastic adherence (PA-MSCs).
Methods: The in vitro model of wound healing (CytoSelect™ 24-Well Wound Healing Assay) was used in order to compare the migration and proliferation potential of CD271-MSCs and PA-MSCs of passage 2 and 4 cultured in presence or absence of growth factors or cytokines.
Results: CD271-MSCs of both passages when compared to PA-MSCs demonstrated a significantly higher potential to close the wound 12 and 24 h after initiation of the wound healing assay (P < 0.003 and P < 0.002, respectively). Noteworthy, the migration capacity of PA-MSCs of second passage was significantly improved after stimulation with FGF-2 (P < 0.02), PDGF-BB (P < 0.006), MCP-1 (P < 0.002) and IL-6 (P < 0.03), whereas only TGF-β enhanced significantly migration process of PA-MSCs of P4 12 h after the treatment (P < 0.02). Interestingly, treatment of CD271-MSCs of both passages with growth factors or cytokines did not affect their migratory potential.
Conclusions: Our in vitro data provide the first evidence that CD271-MSCs are significantly more potent in "wound healing" than their counterparts PA-MSCs.
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Metadaten
Author:Hatixhe Latifi‑Pupovci, Zyrafete Kuçi, Sibylle Wehner, Halvard-Björn Bönig, Ralf Lieberz, Thomas Klingebiel, Peter Bader, Selim Kuçi
URN:urn:nbn:de:hebis:30:3-506003
DOI:http://dx.doi.org/10.1186/s12967-015-0676-9
ISSN:1479-5876
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=26407865
Parent Title (English):Journal of translational medicine
Publisher:BioMed Central
Place of publication:London
Document Type:Article
Language:English
Year of Completion:2015
Date of first Publication:2015/09/25
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/07/01
Tag:Bone marrow; MSC-subsets; Wound healing potential
Volume:13
Issue:Art. 315
Pagenumber:9
First Page:1
Last Page:9
Note:
Open Access: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
HeBIS PPN:450688585
Institutes:Medizin
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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