Time- and polarity-dependent proteomic changes associated with homeostatic scaling at central synapses

In homeostatic scaling at central synapses, the depth and breadth of cellular mechanisms that detect the offset from the set-point, detect the duration of the offset and implement a cellular response are not well underst
In homeostatic scaling at central synapses, the depth and breadth of cellular mechanisms that detect the offset from the set-point, detect the duration of the offset and implement a cellular response are not well understood. To understand the time-dependent scaling dynamics we treated cultured rat hippocampal cells with either TTX or bicucculline for 2 hr to induce the process of up- or down-scaling, respectively. During the activity manipulation we metabolically labeled newly synthesized proteins using BONCAT. We identified 168 newly synthesized proteins that exhibited significant changes in expression. To obtain a temporal trajectory of the response, we compared the proteins synthesized within 2 hr or 24 hr of the activity manipulation. Surprisingly, there was little overlap in the significantly regulated newly synthesized proteins identified in the early- and integrated late response datasets. There was, however, overlap in the functional categories that are modulated early and late. These data indicate that within protein function groups, different proteomic choices can be made to effect early and late homeostatic responses that detect the duration and polarity of the activity manipulation.
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Metadaten
Author:Christoph Tobias Schanzenbächer, Julian David Langer, Erin Schuman
URN:urn:nbn:de:hebis:30:3-510083
DOI:http://dx.doi.org/10.7554/eLife.33322.001
ISSN:2050-084X
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=29447110
Parent Title (English):eLife
Publisher:eLife Sciences Publications
Place of publication:Cambridge
Contributor(s):Yukiko Goda
Document Type:Article
Language:English
Year of Completion:2018
Date of first Publication:2018/02/15
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/09/05
Tag:Cell biology; Research article; homeostasis; neuroscience; proteomics; rat; synaptic plasticity; synaptic scaling
Volume:7
Issue:e33322
Pagenumber:20
First Page:1
Last Page:20
Note:
Copyright Schanzenbächer et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
HeBIS PPN:453726445
Institutes:Biowissenschaften
MPI für Hirnforschung
Dewey Decimal Classification:570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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