- hallmark of ageing is the redistribution of body fat. Particularly, subcutaneous fat decreases paralleled by a decrease of skin collagen I are typical for age-related skin atrophy. In this paper, we hypothesize that collagen I may be a relevant molecule stimulating the differentiation of adipose-derived stem cells (ASCs) into adipocytes augmenting subcutaneous fat. In this context lipogenesis, adiponectin, and collagen I receptor expression were determined. Freshly isolated ASCs were characterized by stemness-associated surface markers by FACS analysis and then transdifferentiated into adipocytes by specific medium supplements. Lipogenesis was evaluated using Nile Red staining and documented by fluorescence microscopy or quantitatively measured by using a multiwell spectrofluorometer. Expression of adiponectin was measured by real-time RT-PCR and in cell-free supernatants by ELISA, and expression of collagen I receptors was observed by western blot analysis. It was found that supports coated with collagen I promote cell adhesion and lipogenesis of ASCs. Interestingly, a reverse correlation to adiponectin expression was observed. Moreover, we found upregulation of the collagen receptor, discoidin domain-containing receptor 2; receptors of the integrin family were absent or downregulated. These findings indicate that collagen I is able to modulate lipogenesis and adiponectin expression and therefore may contribute to metabolic dysfunctions associated with ageing.
MetadatenAuthor: | Nadja Nicole ZöllerORCiDGND, Sarah Schreiner, Laura Petry, Stephanie Hoffmann, Katja Steinhorst, Johannes KleemannORCiDGND, Manuel Alexander JägerGND, Roland KaufmannGND, Markus MeissnerORCiDGND, Stefan KippenbergerORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-510136 |
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DOI: | https://doi.org/10.3390/cells8040302 |
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ISSN: | 2073-4409 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/30939867 |
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Parent Title (English): | Cells |
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Publisher: | MDPI |
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Place of publication: | Basel |
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Document Type: | Article |
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Language: | English |
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Year of Completion: | 2019 |
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Date of first Publication: | 2019/04/01 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2019/09/12 |
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Tag: | adipocytes; adiponectin; adipose-derived stem cells; ageing; collagen I; differentiation; discoidin domain-containing receptor; integrins; subcutaneous fat |
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Volume: | 8 |
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Issue: | 4, Art. 302 |
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Page Number: | 13 |
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First Page: | 1 |
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Last Page: | 13 |
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Note: | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
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HeBIS-PPN: | 454003528 |
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Institutes: | Medizin / Medizin |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Open-Access-Publikationsfonds: | Medizin |
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Licence (German): | Creative Commons - Namensnennung 4.0 |
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