Long and repeat-rich intronic sequences favor circular rna formation under conditions of reduced spliceosome activity

Circular RNAs (circRNAs), an important class of regulatory RNAs, have been shown to be the most prevalent in the brain compared with other tissues. However the processes governing their biogenesis in neurons are still el
Circular RNAs (circRNAs), an important class of regulatory RNAs, have been shown to be the most prevalent in the brain compared with other tissues. However the processes governing their biogenesis in neurons are still elusive. Moreover, little is known about whether and how different biogenesis factors work in synchrony to generate neuronal circRNAs. To address this question, we pharmacologically inhibited the spliceosome and profiled rat neuronal circRNAs using RNA sequencing. We identified over 100 circRNAs that were up-regulated and a few circRNAs that were down-regulated upon spliceosome inhibition. Bioinformatic analysis revealed that up-regulated circRNAs possess significantly longer flanking introns compared with the un-changed circRNA population. Moreover, the flanking introns of up-regulated circRNAs harbor a higher number of distinct repeat sequences and more reverse complementary motifs compared with the unchanged circRNAs. Taken together, our data demonstrate that the biogenesis of circRNAs containing distinct intronic features becomes favored under conditions of limited spliceosome activity.
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Metadaten
Author:Mantian Wang, Jingyi Hou, Michaela Müller-McNicoll, Wei Chen, Erin Schuman
URN:urn:nbn:de:hebis:30:3-514269
DOI:http://dx.doi.org/10.1016/j.isci.2019.08.058
ISSN:2589-0042
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=31590076
Parent Title (English):iScience
Publisher:Elsevier
Place of publication:Amsterdam ; Bosten ; London ; New York ; Oxford ; Paris ; Philadelphia ; San Diego ; St. Louis
Document Type:Article
Language:English
Year of Completion:2019
Date of first Publication:2019/09/06
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/10/21
Tag:Biological Sciences; Cell Biology; Molecular Biology; Omics
Volume:20
Pagenumber:22
First Page:237
Last Page:247
Note:
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
HeBIS PPN:455705364
Institutes:Biowissenschaften
Dewey Decimal Classification:570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell - Keine Bearbeitung 4.0

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