Flotillins regulate focal adhesions by interacting with α-actinin and by influencing the activation of focal adhesion kinase
- Cell–matrix adhesion and cell migration are physiologically important processes that also play a major role in cancer spreading. In cultured cells, matrix adhesion depends on integrin-containing contacts such as focal adhesions. Flotillin-1 and flotillin-2 are frequently overexpressed in cancers and are associated with poor survival. Our previous studies have revealed a role for flotillin-2 in cell–matrix adhesion and in the regulation of the actin cytoskeleton. We here show that flotillins are important for cell migration in a wound healing assay and influence the morphology and dynamics of focal adhesions. Furthermore, anchorage-independent growth in soft agar is enhanced by flotillins. In the absence of flotillins, especially flotillin-2, phosphorylation of focal adhesion kinase and extracellularly regulated kinase is diminished. Flotillins interact with α-actinin, a major regulator of focal adhesion dynamics. These findings are important for understanding the molecular mechanisms of how flotillin overexpression in cancers may affect cell migration and, especially, enhance metastasis formation.
Author: | Antje Banning, Tanja Babuke, Nina Susanne KurrleORCiDGND, Melanie Meister, Mika O. Ruonala, Ritva TikkanenORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-515182 |
DOI: | https://doi.org/10.3390/cells7040028 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/29642469 |
Parent Title (English): | Cells |
Publisher: | MDPI |
Place of publication: | Basel |
Document Type: | Article |
Language: | English |
Year of Completion: | 2018 |
Date of first Publication: | 2018/04/07 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2019/10/24 |
Tag: | cancer; cell migration; focal adhesion; metastasis |
Volume: | 7 |
Issue: | 28 |
Page Number: | 20 |
Note: | © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
HeBIS-PPN: | 455331847 |
Institutes: | Biowissenschaften / Biowissenschaften |
Medizin / Medizin | |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit | |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung 4.0 |