Genome-based analysis of the transcriptome from mature chickpea root nodules

Symbiotic nitrogen fixation (SNF) in root nodules of grain legumes such as chickpea is a highly complex process that drastically affects the gene expression patterns of both the prokaryotic as well as eukaryotic interact
Symbiotic nitrogen fixation (SNF) in root nodules of grain legumes such as chickpea is a highly complex process that drastically affects the gene expression patterns of both the prokaryotic as well as eukaryotic interacting cells. A successfully established symbiotic relationship requires mutual signaling mechanisms and a continuous adaptation of the metabolism of the involved cells to varying environmental conditions. Although some of these processes are well understood today many of the molecular mechanisms underlying SNF, especially in chickpea, remain unclear. Here, we reannotated our previously published transcriptome data generated by deepSuperSAGE (Serial Analysis of Gene Expression) to the recently published draft genome of chickpea to assess the root- and nodule-specific transcriptomes of the eukaryotic host cells. The identified gene expression patterns comprise up to 71 significantly differentially expressed genes and the expression of twenty of these was validated by quantitative real-time PCR with the tissues from five independent biological replicates. Many of the differentially expressed transcripts were found to encode proteins implicated in sugar metabolism, antioxidant defense as well as biotic and abiotic stress responses of the host cells, and some of them were already known to contribute to SNF in other legumes. The differentially expressed genes identified in this study represent candidates that can be used for further characterization of the complex molecular mechanisms underlying SNF in chickpea.
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Metadaten
Author:Fabian Afonso-Grunz, Carlos Molina, Klaus Hoffmeier, Lukas Rycak, Himabindu Kudapa, Rajeev K. Varshney, Jean-Jacques Drevon, Peter Winter, Günter Kahl
URN:urn:nbn:de:hebis:30:3-515435
DOI:http://dx.doi.org/10.3389/fpls.2014.00325
Parent Title (English):Frontiers in plant science
Publisher:Frontiers Research Foundation
Place of publication:Lausanne
Document Type:Article
Language:English
Year of Completion:2014
Date of first Publication:2014/07/11
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/10/22
Tag:Cicer arietinum; chickpea genome sequence; deepSuperSAGE; gene expression profiling; root nodules; symbiotic nitrogen fixation
Volume:5
Issue:325
Note:
Copyright © 2014 Afonso-Grunz, Molina, Hoffmeier, Rycak, Kudapa, Varshney, Drevon, Winter and Kahl. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) http://creativecommons.org/licenses/by/3.0/ . The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
HeBIS PPN:455355576
Institutes:Biowissenschaften
Dewey Decimal Classification:580 Pflanzen (Botanik)
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 3.0

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