An ancestral apical brain region contributes to the central complex under the control of foxQ2 in the beetle Tribolium

The genetic control of anterior brain development is highly conserved throughout animals. For instance, a conserved anterior gene regulatory network specifies the ancestral neuroendocrine center of animals and the apical
The genetic control of anterior brain development is highly conserved throughout animals. For instance, a conserved anterior gene regulatory network specifies the ancestral neuroendocrine center of animals and the apical organ of marine organisms. However, its contribution to the brain in non-marine animals has remained elusive. Here, we study the function of the Tc-foxQ2 forkhead transcription factor, a key regulator of the anterior gene regulatory network of insects. We characterized four distinct types of Tc-foxQ2 positive neural progenitor cells based on differential co-expression with Tc-six3/optix, Tc-six4, Tc-chx/vsx, Tc-nkx2.1/scro, Tcey, Tc-rx and Tc-fez1. An enhancer trap line built by genome editing marked Tc-foxQ2 positive neurons, which projected through the primary brain commissure and later through a subset of commissural fascicles. Eventually, they contributed to the central complex. Strikingly, in Tc-foxQ2 RNAi knock-down embryos the primary brain commissure did not split and subsequent development of midline brain structures stalled. Our work establishes foxQ2 as a key regulator of brain midline structures, which distinguish the protocerebrum from segmental ganglia. Unexpectedly, our data suggest that the central complex evolved by integrating neural cells from an ancestral anterior neuroendocrine center.
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Metadaten
Author:Bicheng He, Marita Büscher, Max Stephen Farnworth, Frederic Strobl, Ernst H. K. Stelzer, Nikolaus Dieter Bernhard Koniszewski, Dominik Mühlen, Gregor Bucher
URN:urn:nbn:de:hebis:30:3-531258
DOI:http://dx.doi.org/10.7554/eLife.49065
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=31625505
Parent Title (English):eLife
Document Type:Article
Language:English
Date of Publication (online):2019/10/18
Date of first Publication:2019/10/18
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/04/09
Volume:8
Issue:e49065
Pagenumber:29
First Page:1
Last Page:29
Note:
(c) Copyright He et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
HeBIS PPN:463773152
Institutes:Biowissenschaften
Dewey Decimal Classification:570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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