Neuronal activity triggers uptake of hematopoietic extracellular vesicles in vivo

Communication with the hematopoietic system is a vital component of regulating brain function in health and disease. Traditionally, the major routes considered for this neuroimmune communication are by individual molecul
Communication with the hematopoietic system is a vital component of regulating brain function in health and disease. Traditionally, the major routes considered for this neuroimmune communication are by individual molecules such as cytokines carried by blood, by neural transmission, or, in more severe pathologies, by the entry of peripheral immune cells into the brain. In addition, functional mRNA from peripheral blood can be directly transferred to neurons via extracellular vesicles (EVs), but the parameters that determine their uptake are unknown. Using varied animal models that stimulate neuronal activity by peripheral inflammation, optogenetics, and selective proteasome inhibition of dopaminergic (DA) neurons, we show that the transfer of EVs from blood is triggered by neuronal activity in vivo. Importantly, this transfer occurs not only in pathological stimulation but also by neuronal activation caused by the physiological stimulus of novel object placement. This discovery suggests a continuous role of EVs under pathological conditions as well as during routine cognitive tasks in the healthy brain.
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Metadaten
Author:Ivan-Maximiliano Kur, Pierre-Hugues Prouvot, Ting Fu, Wei Fan, Felicia Müller-Braun, Avash Das, Saumya Das, Thomas Deller, Jochen Roeper, Albrecht Stroh, Stefan Momma
URN:urn:nbn:de:hebis:30:3-531348
DOI:http://dx.doi.org/10.1371/journal.pbio.3000643
ISSN:1545-7885
ISSN:1544-9173
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=32176686
Parent Title (English):PLoS biology
Publisher:PLoS
Place of publication:Lawrence, KS
Contributor(s):Hugo J. Bellen
Document Type:Article
Language:English
Year of Completion:2020
Date of first Publication:2020/03/16
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/03/18
Tag:Gene expression; Intravenous injections; Marker genes; Microglial cells; Mouse models; Neurons; Optogenetics; Yellow fluorescent protein
Volume:18
Issue:(3): e3000643
Pagenumber:15
First Page:1
Last Page:15
Note:
Copyright: © 2020 Kur et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
HeBIS PPN:463913086
Institutes:Biowissenschaften
Medizin
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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