The testicular soma of Tsc22d3 knockout mice supports spermatogenesis and germline transmission from spermatogonial stem cell lines upon transplantation

Spermatogonial stem cells (SSCs) are adult stem cells that are slowly cycling and self-renewing. The pool of SSCs generates very large numbers of male gametes throughout the life of the individual. SSCs can be cultured i
Spermatogonial stem cells (SSCs) are adult stem cells that are slowly cycling and self-renewing. The pool of SSCs generates very large numbers of male gametes throughout the life of the individual. SSCs can be cultured in vitro for long periods of time, and established SSC lines can be manipulated genetically. Upon transplantation into the testes of infertile mice, long-term cultured mouse SSCs can differentiate into fertile spermatozoa, which can give rise to live offspring. Here, we show that the testicular soma of mice with a conditional knockout (conKO) in the X-linked gene Tsc22d3 supports spermatogenesis and germline transmission from cultured mouse SSCs upon transplantation. Infertile males were produced by crossing homozygous Tsc22d3 floxed females with homozygous ROSA26-Cre males. We obtained 96 live offspring from six long-term cultured SSC lines with the aid of intracytoplasmic sperm injection. We advocate the further optimization of Tsc22d3-conKO males as recipients for testis transplantation of SSC lines.
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Author:Hai Zhou, Zhen Zeng, Frank Köntgen, Mona Khan, Peter Mombaerts
URN:urn:nbn:de:hebis:30:3-533218
DOI:http://dx.doi.org/10.1002/dvg.23295
ISSN:1526-968X
ISSN:1526-954X
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=31001916
Parent Title (English):Genesis
Publisher:Wiley
Place of publication:Chichester [u. a.]
Document Type:Article
Language:English
Year of Completion:2019
Date of first Publication:2019/04/19
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/03/25
Tag:GILZ; SSC; germline stem cell; spermatogenesis
Volume:57
Issue:6, e23295
Pagenumber:10
First Page:1
Last Page:10
Note:
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Institutes:Interdisziplinäres Zentrum für Neurowissenschaften Frankfurt (IZNF)
MPI für Hirnforschung
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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