Characterization of the cellular reaction to a collagen-based matrix: an in vivo histological and histomorphometrical analysis

The permeability and inflammatory tissue reaction to Mucomaix® matrix (MM), a non- cross-linked collagen-based matrix was evaluated in both ex vivo and in vivo settings. Liquid platelet rich fibrin (PRF), a blood concent
The permeability and inflammatory tissue reaction to Mucomaix® matrix (MM), a non- cross-linked collagen-based matrix was evaluated in both ex vivo and in vivo settings. Liquid platelet rich fibrin (PRF), a blood concentrate system, was used to assess its capacity to absorb human proteins and interact with blood cells ex vivo. In the in vivo aspect, 12 Wister rats had MM implanted subcutaneously, whereas another 12 rats (control) were sham-operated without biomaterial implantation. On days 3, 15 and 30, explantation was completed (four rats per time-point) to evaluate the tissue reactions to the matrix. Data collected were statistically analyzed using analysis of variance (ANOVA) and Tukey multiple comparisons tests (GraphPad Prism 8). The matrix absorbed the liquid PRF in the ex vivo study. Day 3 post-implantation revealed mild tissue inflammatory reaction with presence of mononuclear cells in the implantation site and on the biomaterial surface (mostly CD68-positive macrophages). The control group at this stage had more mononuclear cells than the test group. From day 15, multinucleated giant cells (MNGCs) were seen in the implantation site and the outer third of the matrix with marked increase on day 30 and spread to the matrix core. The presence of these CD68-positive MNGCs was associated with significant matrix vascularization. The matrix degraded significantly over the study period, but its core was still visible as of day 30 post-implantation. The high permeability and fast degradation properties of MM were highlighted.
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Metadaten
Author:Samuel Ebele Udeabor, Carlos Herrera-Vizcaíno, Robert Alexander Sader, James Kirkpatrick, Sarah Al-Maawi, Shahram Ghanaati
URN:urn:nbn:de:hebis:30:3-547199
DOI:http://dx.doi.org/10.3390/ma13122730
ISSN:1996-1944
Parent Title (English):Materials
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Date of Publication (online):2020/06/16
Date of first Publication:2020/06/16
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/06/18
Tag:Mucomaix® matrix; cellular reaction; collagen-based matrix; histology; histomorphometry; liquid PRF
Volume:13
Issue:2730
Pagenumber:16
HeBIS PPN:467141061
Institutes:Medizin
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Open-Access-Publikationsfonds:Medizin
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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