Role of bioelectricity during cell proliferation in different cell types

Most living organisms possess varying degrees of regenerative capabilities but how these regenerative processes are controlled is still poorly understood. Naturally occurring bioelectric voltages (like Vmem) are thought 
Most living organisms possess varying degrees of regenerative capabilities but how these regenerative processes are controlled is still poorly understood. Naturally occurring bioelectric voltages (like Vmem) are thought to be playing instructive role in tissue regeneration, as well as embryonic development. The different distribution of ions on the either side of the cell membrane results in intra- and extra-cellular voltage differences, known as membrane potential or Vmem. The relationship between Vmem and cell physiology is conserved in a wide range of cell types and suggests that Vmem regulation is a fundamental control mechanism for regeneration related processes e.g., proliferation and differentiation. In the present study we measured Vmem in three different cell types (human osteogenic sarcoma cell line (OSC), rat bone marrow derived mesenchymal stem cells (BM-MSC), and rat dermal fibroblasts) and characterized the relationship between their Vmem and proliferation. In order to find out if Vmem controls proliferation, or visa-versa, we blocked and then unblocked Na+/K+-exchanging ATPase using ouabain and measured the proliferation. Our results demonstrate that Vmem can be pharmacologically manipulated to control proliferation in certain cell types like BM-MSC. Taken together, it is clear that control of bioelectrical properties in non-excitable cells could prove to be potentially a useful tool in regenerative medicine efforts.
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Metadaten
Author:Mit Balvantray Bhavsar, Liudmila Leppik, Karla Mychellyne Costa Oliveira, John H. Barker
URN:urn:nbn:de:hebis:30:3-550558
DOI:http://dx.doi.org/10.3389/fbioe.2020.00603
ISSN:2296-4185
Parent Title (English):Frontiers in bioengineering and biotechnology
Publisher:Frontiers Media S.A.
Place of publication:Lausanne
Document Type:Article
Language:English
Date of Publication (online):2020/07/02
Date of first Publication:2020/07/02
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/07/02
Tag:Vmem; cell proliferation; fibroblasts; membrane potential; mesenchymal stem cell; osteogenic sarcoma cells; ouabain
Volume:8
Issue:603
Pagenumber:9
HeBIS PPN:467136971
Institutes:Medizin
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Open-Access-Publikationsfonds:Medizin
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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