Recent advances in practical methods for liver cell biology: a short overview

Molecular and cellular research modalities for the study of liver pathologies have been tremendously improved over the recent decades. Advanced technologies offer novel opportunities to establish cell isolation technique
Molecular and cellular research modalities for the study of liver pathologies have been tremendously improved over the recent decades. Advanced technologies offer novel opportunities to establish cell isolation techniques with excellent purity, paving the path for 2D and 3D microscopy and high-throughput assays (e.g., bulk or single-cell RNA sequencing). The use of stem cell and organoid research will help to decipher the pathophysiology of liver diseases and the interaction between various parenchymal and non-parenchymal liver cells. Furthermore, sophisticated animal models of liver disease allow for the in vivo assessment of fibrogenesis, portal hypertension and hepatocellular carcinoma (HCC) and for the preclinical testing of therapeutic strategies. The purpose of this review is to portray in detail novel in vitro and in vivo methods for the study of liver cell biology that had been presented at the workshop of the 8th meeting of the European Club for Liver Cell Biology (ECLCB-8) in October of 2018 in Bonn, Germany.
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Metadaten
Author:Sandra Torres, Zeinab Abdullah, Maximilian J. Brol, Claus Hellerbrand, Mercedes Fernandez, Romina Fiorotto, Sabine Klein, Philipp Königshofer, Christian Liedtke, Sophie Lotersztajn, Yulia A. Nevzorova, Robert Schierwagen, Thomas Reiberger, Frank Erhard Uschner, Frank Tacke, Ralf Weiskirchen, Jonel Trebicka
URN:urn:nbn:de:hebis:30:3-552956
DOI:http://dx.doi.org/10.3390/ijms21062027
ISSN:1422-0067
ISSN:1661-6596
Parent Title (English):International journal of molecular sciences
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Date of Publication (online):2020/03/16
Date of first Publication:2020/03/16
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/08/06
Tag:hepatic stellate cells; hepatocellular cancer; in vitro models; steatosis; fibrogenesis
Volume:21
Issue:(6), 2027
Pagenumber:19
First Page:1
Last Page:19
Note:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
HeBIS PPN:469733608
Institutes:Medizin
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Open-Access-Publikationsfonds:Medizin
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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