Comparative analysis of phenotypic and genotypic antibiotic susceptibility patterns in Mycobacterium avium complex

Objective: Phenotypic (Sensititre Myco, pDST) and genotypic drug susceptibility testing (GenoType NTM DR, gDST) in M. avium complex (MAC) have become available as standardized assays, but comparable data is needed. This 
Objective: Phenotypic (Sensititre Myco, pDST) and genotypic drug susceptibility testing (GenoType NTM DR, gDST) in M. avium complex (MAC) have become available as standardized assays, but comparable data is needed. This study aimed to investigate the phenotypic and genotypic drug susceptibility patterns in MAC clinical isolates.
Methods: Overall, 98 isolates from 85 patients were included. pDST and gDST were performed on all isolates and results compared regarding specificity and sensitivity using pDST as a reference method. The impact of drug instability on pDST results was studied using a biological assay over 14 days. In addition, the evolution of antimicrobial resistance was investigated in sequential isolates of 13 patients.
Results: Macrolide resistance was rare, 1.2% (95% CI 0.7–7.3) of isolates in the base cohort. No aminoglycoside resistances were found, but 14.1% of the studied isolates (95% CI 7.8–23.8) showed intermediate susceptibility. The GenoType NTM DR identified two out of four macrolide-resistant isolates. Antibiotic stability was demonstrated to be poor in rifampicin, rifabutin, and doxycycylin.
Conclusions: pDST results in NTM for unstable antibiotics must be interpreted with care. A combination of pDST and gDST will be useful for the guidance of antimicrobial therapy in MAC-disease.
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Metadaten
Author:Nils Wetzstein, Thomas A. Kohl, Sönke Andres, Tilman G. Schultze, Ari Geil, Eunhee Kim, Teodora Biciusca, Christian Hügel, Michael Hogardt, Annette Lehn, Maria J. G. T. Vehreschild, Timo Wolf, Stefan Niemann, Florian P. Maurer, Thomas Alexander Wichelhaus
URN:urn:nbn:de:hebis:30:3-552965
DOI:http://dx.doi.org/10.1016/j.ijid.2020.02.059
ISSN:1201-9712
ISSN:1878-3511
Parent Title (English):International journal of infectious diseases
Publisher:Elsevier
Place of publication:Amsterdam
Document Type:Article
Language:English
Date of Publication (online):2020/03/05
Date of first Publication:2020/03/05
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/08/06
Tag:DST; Drug susceptibility testing; M. Intracellulare; M. avium; M. avium complex; M. chimaera; NTM; Non-tuberculous mycobacteria
Volume:93
Pagenumber:9
First Page:320
Last Page:328
Note:
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Institutes:Medizin
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Open-Access-Publikationsfonds:Medizin
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell - Keine Bearbeitung 4.0

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