Light‐induced uncaging for time‐resolved observations of biochemical reactions by MAS NMR spectroscopy

Light‐induced activation of biomolecules by uncaging of photolabile protection groups has found many applications for triggering biochemical reactions with minimal perturbations directly within cells. Such an approach mi
Light‐induced activation of biomolecules by uncaging of photolabile protection groups has found many applications for triggering biochemical reactions with minimal perturbations directly within cells. Such an approach might also offer unique advantages for solid‐state NMR experiments on membrane proteins for initiating reactions within or at the membrane directly within the closed MAS rotor. Herein, we demonstrate that the integral membrane protein E. coli diacylglycerol kinase (DgkA), which catalyzes the phosphorylation of diacylglycerol, can be controlled by light under MAS‐NMR conditions. Uncaging of NPE‐ATP or of lipid substrate NPE‐DOG by in situ illumination triggers its enzymatic activity, which can be monitored by real‐time 31P‐MAS NMR. This proof‐of‐concept illustrates that combining MAS‐NMR with uncaging strategies and illumination methods offers new possibilities for controlling biochemical reactions at or within lipid bilayers.
show moreshow less

Export metadata

  • Export Bibtex
  • Export RIS

Additional Services

    Share in Twitter Search Google Scholar
Metadaten
Author:Julian Martinus de Mos, Andreas Jakob, Johanna Becker-Baldus, Alexander Heckel, Clemens Glaubitz
URN:urn:nbn:de:hebis:30:3-565081
DOI:http://dx.doi.org/10.1002/chem.202000770
ISSN:1521-3765
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=32240561
Parent Title (German):Chemistry - a European journal
Publisher:Wiley-VCH
Place of publication:Weinheim
Document Type:Article
Language:English
Date of Publication (online):2020/05/13
Date of first Publication:2020/04/02
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/11/11
Tag:DgkA; NMR spectroscopy; caged ATP; caged diacylglycerol; enzymes; solid-state NMR
Volume:26
Issue:30
Pagenumber:4
First Page:6789
Last Page:6792
HeBIS PPN: 476868890
Institutes:Biochemie und Chemie
Dewey Decimal Classification:540 Chemie und zugeordnete Wissenschaften
570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0

$Rev: 11761 $