A practical synthesis of the 13C/15N-labelled tripeptide N-formyl-Met-Leu-Phe, useful as a reference in solid-state NMR spectroscopy

A mild synthetic method for N-formyl-Met-Leu-Phe-OH (1) is described. After Fmoc solid phase peptide synthesis, on-bead formylation and HPLC purification, more than 30 mg of the fully 13C/15N-labelled tripeptide 1 could be isolated in a typical batch. This peptide can be easily crystallised and is therefore well suited as a standard sample for setting up solid-state NMR experiments.

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Metadaten
Author:Sven Thomas Breitung, Jakob J. Lopez, Gerd Dürner, Clemens Glaubitz, Michael W. Göbel, Marcel Suhartono
URN:urn:nbn:de:hebis:30-60239
Document Type:Article
Language:English
Date of Publication (online):12.11.2008
Year of first Publication:2008
Publishing Institution:Univ.-Bibliothek Frankfurt am Main
Tag:Fmoc solid phase peptide synthesis ; Wang resin; f-MLF ; formylation ; magic-angle spinning
Source:Beilstein Journal of Organic Chemistry 2008; 4:35. ; doi:10.3762/bjoc.4.35
HeBIS PPN:208593497
Institutes:Biochemie und Chemie
Dewey Decimal Classification:540 Chemie und zugeordnete Wissenschaften
Sammlungen:Universitätspublikationen
Note:
This is an Open Access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 

The license is subject to the Beilstein Journal of Organic Chemistry terms and conditions: (http://www.beilstein-journals.org/bjoc)

The definitive version of this article is the electronic one which can be found at: doi:10.3762/bjoc.4.35
Licence (German):License LogoCreative Commons - Namensnennung 2.0

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