OXA-484, an OXA-48-Type Carbapenem-Hydrolyzing Class D β-Lactamase From Escherichia coli
- OXA-48-like carbapenemases are among the most frequent carbapenemases in Gram-negative Enterobacterales worldwide with the highest prevalence in the Middle East, North Africa and Europe. Here, we investigated the so far uncharacterized carbapenemase OXA-484 from a clinical E. coli isolate belonging to the high-risk clone ST410 regarding antibiotic resistance pattern, horizontal gene transfer (HGT) and genetic support. OXA-484 differs by the amino acid substitution 214G compared to the most closely related variants OXA-181 (214R) and OXA-232 (214S). The blaOXA–484 was carried on a self-transmissible 51.5 kb IncX3 plasmid (pOXA-484) showing high sequence similarity with plasmids harboring blaOXA–181. Intraspecies and intergenus HGT of pOXA-484 to different recipients occurred at low frequencies of 1.4 × 10–7 to 2.1 × 10–6. OXA-484 increased MICs of temocillin and carbapenems similar to OXA-232 and OXA-244, but lower compared with OXA-48 and OXA-181. Hence, OXA-484 combines properties of OXA-181-like plasmid support and transferability as well as β-lactamase activity of OXA-232.
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| Author: | Julian Sommer, Kristina M. Gerbracht, Felix F. Krause, Florian Wild, Manuela TietgenORCiDGND, Sara Riedel-Christ, Janko Sattler, Axel HamprechtORCiDGND, Volkhard A. J. KempfORCiDGND, Stephan GöttigORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-612526 |
| DOI: | https://doi.org/10.3389/fmicb.2021.660094 |
| ISSN: | 1664-302X |
| Parent Title (English): | Frontiers in microbiology |
| Publisher: | Frontiers Media |
| Place of publication: | Lausanne |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/05/12 |
| Date of first Publication: | 2021/05/12 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2021/06/30 |
| Tag: | Enterobacterales; plasmid IncX; OXA-48; OXA-484; beta-lactamases; carbapenemases |
| Volume: | 12 |
| Issue: | art. 660094 |
| Page Number: | 9 |
| First Page: | 1 |
| Last Page: | 9 |
| HeBIS-PPN: | 484966367 |
| Institutes: | Medizin |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Sammlungen: | Universitätspublikationen |
| Open-Access-Publikationsfonds: | Medizin |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
