Developmental and heat stress-regulated expression of HsfA2 and small heat shock proteins in tomato anthers
- The high sensitivity of male reproductive cells to high temperatures may be due to an inadequate heat stress response. The results of a comprehensive expression analysis of HsfA2 and Hsp17-CII, two important members of the heat stress system, in the developing anthers of a heat-tolerant tomato genotype are reported here. A transcriptional analysis at different developmental anther/pollen stages was performed using semi-quantitative and real-time PCR. The messengers were localized using in situ RNA hybridization, and protein accumulation was monitored using immunoblot analysis. Based on the analysis of the gene and protein expression profiles, HsfA2 and Hsp17-CII are finely regulated during anther development and are further induced under both short and prolonged heat stress conditions. These data suggest that HsfA2 may be directly involved in the activation of protection mechanisms in the tomato anther during heat stress and, thereby, may contribute to tomato fruit set under adverse temperatures. Key words: Anther development, heat stress, HsfA2, Hsp17-CII, pollen, tomato
Author: | Filomena Giorno, Mieke Wolters-Arts, Stefania Grillo, Klaus-Dieter Scharf, Wim H. Vriezen, Celestina Mariani |
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URN: | urn:nbn:de:hebis:30-73387 |
DOI: | https://doi.org/10.1093/jxb/erp316 |
ISSN: | 1460-2431 |
ISSN: | 0022-0957 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/19854799 |
Parent Title (English): | The journal of experimental botany |
Publisher: | Oxford Univ. Press |
Place of publication: | Oxford |
Document Type: | Article |
Language: | English |
Year of Completion: | 2009 |
Date of first Publication: | 2009/10/23 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2009/12/28 |
Tag: | HsfA2; Hsp17-CII; anther development; heat stress; pollen; tomato |
Volume: | 61 |
Issue: | 2 |
Page Number: | 10 |
First Page: | 453 |
Last Page: | 462 |
Note: | © 2009 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details) |
Source: | Journal of Experimental Botany ; doi:10.1093/jxb/erp316 |
HeBIS-PPN: | 220572739 |
Institutes: | Biowissenschaften / Biowissenschaften |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik) |
Sammlungen: | Sammlung Biologie / Sondersammelgebiets-Volltexte |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell 3.0 |