Genome sequence of the necrotrophic plant pathogen Pythium ultimum reveals original pathogenicity mechanisms and effector repertoire

Background: Pythium ultimum (P. ultimum) is a ubiquitous oomycete plant pathogen responsible for a variety of diseases on a broad range of crop and ornamental species. Results: The P. ultimum genome (42.8 Mb) encodes 15,
Background: Pythium ultimum (P. ultimum) is a ubiquitous oomycete plant pathogen responsible for a variety of diseases on a broad range of crop and ornamental species. Results: The P. ultimum genome (42.8 Mb) encodes 15,290 genes and has extensive sequence similarity and synteny with related Phytophthora species, including the potato blight pathogen Phytophthora infestans. Whole transcriptome sequencing revealed expression of 86% of genes, with detectable differential expression of suites of genes under abiotic stress and in the presence of a host. The predicted proteome includes a large repertoire of proteins involved in plant pathogen interactions although surprisingly, the P. ultimum genome does not encode any classical RXLR effectors and relatively few Crinkler genes in comparison to related phytopathogenic oomycetes. A lower number of enzymes involved in carbohydrate metabolism were present compared to Phytophthora species, with the notable absence of cutinases, suggesting a significant difference in virulence mechanisms between P. ultimum and more host specific oomycete species. Although we observed a high degree of orthology with Phytophthora genomes, there were novel features of the P. ultimum proteome including an expansion of genes involved in proteolysis and genes unique to Pythium. We identified a small gene family of cadherins, proteins involved in cell adhesion, the first report in a genome outside the metazoans. Conclusions: Access to the P. ultimum genome has revealed not only core pathogenic mechanisms within the oomycetes but also lineage specific genes associated with the alternative virulence and lifestyles found within the pythiaceous lineages compared to the Peronosporaceae.
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Author:C. André Lévesque, Henk Brouwer, Liliana Cano, John P. Hamilton, Carson Holt, Edgar Huitema, Sylvain Raffaele, Gregg P. Robideau, Marco Thines, Joe Win, Marcelo M. Zerillo, Gordon W. Beakes, Jeffrey L. Boore, Dana Busam, Bernard Dumas, Steve Ferriera, Susan I. Fuerstenberg, Claire M. M. Gachon, Elodie Gaulin, Francine Govers, Laura Grenville-Briggs, Neil Horner, Jessica Hostetler, Rays H. Y. Jiang, Justin Johnson, Theerapong Krajaejun, Haining Lin, Harold J. G. Meijer, Barry Moore, Paul Morris, Vipaporn Phuntmart, Daniela Puiu, Jyoti Shetty, Jason E. Stajich, Sucheta Tripathy, Stephan Wawra, Pieter van West, Brett R. Whitty, Pedro M. Coutinho, Bernard Henrissat, Frank Martin, Paul D. Thomas, Brett M. Tyler, Ronald P. De Vries, Sophien Kamoun, Mark Yandell, Ned Tisserat, C. Robin Buell
URN:urn:nbn:de:hebis:30-78353
Document Type:Article
Language:English
Date of Publication (online):2010/08/23
Year of first Publication:2010
Publishing Institution:Univ.-Bibliothek Frankfurt am Main
Release Date:2010/08/23
Note:
© 2010 Lévesque et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Source:Genome Biology 2010, 11:R73 ; doi:10.1186/gb-2010-11-7-r73 ; http://genomebiology.com/2010/11/7/R73/abstract
HeBIS PPN:226357953
Institutes:Institut für Ökologie, Evolution und Diversität
Dewey Decimal Classification:570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung 2.0

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