Endogenous AJAP1 associates with the cytoskeleton and attenuates angiogenesis in endothelial cells
- The adherens junction associated protein 1 (AJAP1, aka shrew-1) is presumably a type-I transmembrane protein localizing and interacting with the E-cadherin-catenin complex. In various tumors, AJAP1 expression is reduced or lost, including hepatocellular and esophageal squamous cell carcinoma, and glial-derived tumors. The aberrant expression of AJAP1 is associated with alterations in cell migration, invasion, increased tumor growth, and tumor vascularization, suggesting AJAP1 as a putative tumor suppressor. We show that AJAP1 attenuates sprouting angiogenesis by reducing endothelial migration and invasion capacities. Further, we show for the first time that endogenous AJAP1 is associated with the microtubule cytoskeleton. This linkage is independent from cell confluency and stable during angiogenic sprouting in vitro. Our work suggests that AJAP1 is a putative negative regulator of angiogenesis, reducing cell migration and invasion by interfering with the microtubule network. Based on our results and those of other authors, we suggest AJAP1 as a novel tumor suppressor and diagnostic marker.
Verfasserangaben: | Katharina Hötte, Isabell Smyrek, Anna Starzinski-PowitzORCiDGND, Ernst H. K. StelzerORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-463487 |
DOI: | https://doi.org/10.1242/bio.022335 |
ISSN: | 2046-6390 |
Pubmed-Id: | https://pubmed.ncbi.nlm.nih.gov/28483980 |
Titel des übergeordneten Werkes (Englisch): | Biology open |
Verlag: | Company |
Verlagsort: | Cambridge |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Jahr der Fertigstellung: | 2017 |
Datum der Erstveröffentlichung: | 15.06.2017 |
Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Datum der Freischaltung: | 26.04.2018 |
Freies Schlagwort / Tag: | AJAP1; Angiogenesis; Cell migration; Cytoskeleton; Endothelial cells; Microtubules; Shrew-1 |
Jahrgang: | 6 |
Ausgabe / Heft: | 6 |
Seitenzahl: | 9 |
Erste Seite: | 723 |
Letzte Seite: | 731 |
Bemerkung: | Copyright & Usage: © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
HeBIS-PPN: | 431860734 |
Institute: | Biowissenschaften / Biowissenschaften |
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe | |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Open-Access-Publikationsfonds: | Biowissenschaften |
Lizenz (Deutsch): | Creative Commons - Namensnennung 3.0 |