Michael Basic, Alica Kubesch, Lisa Kuhnhenn, Esra Görgülü, Fabian Finkelmeier, Julia Dietz, Mate Knabe, Victoria Therese Mücke, Marcus Maximilian Mücke, Annemarie Berger, Stefan Zeuzem, Christoph Sarrazin, Eberhard Hildt, Kai-Henrik Peiffer
- Background & Aims: HBV genotype G (HBV/G) is mainly found in co-infections with other HBV genotypes and was identified as an independent risk factor for liver fibrosis. This study aimed to analyse the prevalence of HBV/G co-infections in healthy European HBV carriers and to characterize the crosstalk of HBV/G with other genotypes.
Methods: A total of 560 European HBV carriers were tested via HBV/G-specific PCR for HBV/G co-infections. Quasispecies distribution was analysed via deep sequencing, and the clinical phenotype was characterized regarding qHBsAg-/HBV-DNA levels and frequent mutations. Replicative capacity and expression of HBsAg/core was studied in hepatoma cells co-expressing HBV/G with either HBV/A, HBV/D or HBV/E using bicistronic vectors.
Results: Although no HBV/G co-infection was found by routine genotyping PCR, HBV/G was detected by specific PCR in 4%-8% of patients infected with either HBV/A or HBV/E but only infrequently in other genotypes. In contrast to HBV/E, HBV/G was found as the quasispecies major variant in co-infections with HBV/A. No differences in the clinical phenotype were observed for HBV/G co-infections. In vitro RNA and DNA levels were comparable among all genotypes, but expression and release of HBsAg was reduced in co-expression of HBV/G with HBV/E. In co-expression with HBV/A and HBV/E expression of HBV/G-specific core was enhanced while core expression from the corresponding genotype was markedly diminished.
Conclusions: HBV/G co-infections are common in European inactive carriers with HBV/A and HBV/E infection, but sufficient detection depends strongly on the assay. HBV/G regulated core expression might play a critical role for survival of HBV/G in co-infections.
MetadatenVerfasserangaben: | Michael Basic, Alica KubeschORCiDGND, Lisa KuhnhennGND, Esra GörgülüORCiDGND, Fabian FinkelmeierORCiDGND, Julia DietzORCiDGND, Mate KnabeORCiD, Victoria Therese MückeORCiDGND, Marcus Maximilian MückeORCiDGND, Annemarie BergerGND, Stefan ZeuzemORCiDGND, Christoph SarrazinGND, Eberhard HildtORCiDGND, Kai-Henrik PeifferORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-618582 |
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DOI: | https://doi.org/10.1111/liv.14884 |
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ISSN: | 1478-3231 |
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Titel des übergeordneten Werkes (Englisch): | Liver international |
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Verlag: | Wiley-Blackwell |
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Verlagsort: | Oxford |
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Dokumentart: | Wissenschaftlicher Artikel |
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Sprache: | Englisch |
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Datum der Veröffentlichung (online): | 31.03.2021 |
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Datum der Erstveröffentlichung: | 31.03.2021 |
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Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Datum der Freischaltung: | 04.08.2021 |
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Freies Schlagwort / Tag: | HBV; co-infection; core expression; genotype G; prevalence |
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Jahrgang: | 41 |
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Ausgabe / Heft: | 6 |
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Seitenzahl: | 12 |
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Erste Seite: | 1278 |
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Letzte Seite: | 1289 |
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HeBIS-PPN: | 489236790 |
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Institute: | Medizin |
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DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Lizenz (Deutsch): | Creative Commons - Namensnennung-Weitergabe unter gleichen Bedingungen 4.0 |
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