Image-guided radiotherapy in an orthotopic mouse model of rectal cancer
- Radiobiology research in rectal cancer has been limited to cell lines, patient-derived organoids (PDOs), or xenografts. Here, we describe a protocol which recapitulates more efficiently the complex contributions of the tumor microenvironment. This approach establishes a preclinical mouse model of rectal cancer by intrarectal transplantation of genetically modified organoids into immunocompetent mice followed by precise image-guided radiotherapy (IGRT) of organoid-induced tumors. This model represents a useful platform to study the cellular and molecular determinants of therapy resistance in rectal cancer.
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| Author: | Adele Mikheal NicolasORCiDGND, Marina PešićGND, Franz RödelORCiDGND, Emmanouil FokasORCiDGND, Florian GretenORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-782987 |
| DOI: | https://doi.org/10.1016/j.xpro.2022.101749 |
| ISSN: | 2666-1667 |
| Parent Title (English): | STAR Protocols |
| Publisher: | Elsevier |
| Place of publication: | Amsterdam |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2022/10/05 |
| Date of first Publication: | 2022/10/05 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2023/10/18 |
| Tag: | Cancer; Health sciences; Model organisms |
| Volume: | 3 |
| Issue: | 4, 101749 |
| Article Number: | 101749 |
| Page Number: | 14 |
| HeBIS-PPN: | 516756389 |
| Institutes: | Medizin |
| Angeschlossene und kooperierende Institutionen / Georg-Speyer-Haus | |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |
