Modeling Hsp70/Hsp40 interaction by multi-scale molecular simulations and coevolutionary sequence analysis
- The interaction between the Heat Shock Proteins 70 and 40 is at the core of the ATPase regulation of the chaperone machinery that maintains protein homeostasis. However, the structural details of the interaction remain elusive and contrasting models have been proposed for the transient Hsp70/Hsp40 complexes. Here we combine molecular simulations based on both coarse-grained and atomistic models with coevolutionary sequence analysis to shed light on this problem by focusing on the bacterial DnaK/DnaJ system. The integration of these complementary approaches resulted in a novel structural model that rationalizes previous experimental observations. We identify an evolutionarily conserved interaction surface formed by helix II of the DnaJ J-domain and a structurally contiguous region of DnaK, involving lobe IIA of the nucleotide binding domain, the inter-domain linker, and the β-basket of the substrate binding domain.
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| Author: | Duccio MalinverniORCiDGND, Alfredo Jost LopezORCiD, Paolo De Los RiosORCiDGND, Gerhard HummerORCiD, Alessandro BarducciORCiD |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-736806 |
| DOI: | https://doi.org/10.7554/eLife.23471 |
| ISSN: | 2050-084X |
| Parent Title (English): | eLife |
| Publisher: | eLife Sciences Publications |
| Place of publication: | Cambridge |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2017/05/12 |
| Date of first Publication: | 2017/05/12 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2023/06/15 |
| Tag: | heat shock protein; molecular modeling; protein coevolution; protein complex; transient interaction |
| Volume: | 6 |
| Issue: | e23471 |
| Page Number: | 20 |
| HeBIS-PPN: | 510031315 |
| Institutes: | Physik |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
