Thermal denaturation equilibria of TMV coat proteins with chemically defined differences in their primary structure
- Thermal denaturation of RNA free coat proteins of tobacco mosaic virus (TMV) was studied for wildtype TMV (vulgare) and the temperature-sensitive mutant, Ni 118. The ability to form soluble aggregates as well as the optical properties (ORD, UV-difference spectra), and the sedimentation behavior were used as criteria for the native state. At pH 7.5, I= 0.02 denaturation is reversible for both proteins. The ORD data indicate that the denatured proteins contain residual secondary structure. The “melting temperatures”, as defined by ORD measurements (cp = 0.02 mM), are 39.5 ± 1°C for vulgare and 27 ± 1°C for Ni 118 at pH 7.5, I= 0.02. By means of the aggregation test (cp = 0.05 mM) somewhat lower melting temperatures were found under the same solvent conditions. The difference between the primary structures of vulgare and Ni 118 proteins being a proline → leucine (pos. 20) replacement, the results suggest the cyclic structure of proline (20) to have a specific stabilizing function in the three dimensional protein structure. This conclusion is supported by preliminary experiments on a temperature-sensitive mutant with a threonine residue in pos. 20.
Author: | Harald JockuschGND, Rudolf KobersteinGND, Rainer JaenickeGND |
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URN: | urn:nbn:de:hebis:30:3-748957 |
DOI: | https://doi.org/10.1515/znb-1969-0527 |
ISSN: | 1865-7117 |
ISSN: | 0932-0776 |
Parent Title (German): | Zeitschrift für Naturforschung, B |
Publisher: | Verlag der Zeitschrift für Naturforschung |
Place of publication: | Tübingen |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2014/06/02 |
Year of first Publication: | 1969 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2023/07/26 |
Volume: | 24 |
Issue: | 5 |
Page Number: | 5 |
First Page: | 613 |
Last Page: | 617 |
HeBIS-PPN: | 512661162 |
Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |